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Neurotrophin Protocols

  • Robert A. Rush

Part of the Methods in Molecular Biology™ book series (MIMB, volume 169)

Table of contents

  1. Front Matter
    Pages i-x
  2. Neurotrophin Quantification and Localization

  3. RNA Quantification and Localization

    1. Front Matter
      Pages 51-51
    2. Edward Chie, Dennis Liu, Xin-Fu Zhou, Robert A. Rush
      Pages 81-90
  4. Construction of Recombinant Proteins,and Transgenic Cells and Animals

    1. Front Matter
      Pages 99-99
    2. Jasodhara Ray, Fred H. Gage
      Pages 115-133
    3. Matthew E. Cunningham, Jan K. Kitajewski, Lloyd A. Greene
      Pages 135-147
  5. In Vivo Analysis

    1. Front Matter
      Pages 193-193
    2. Christopher S. von Bartheld
      Pages 195-216
    3. W. Ruwan Epa, Graham L. Barrett, Perry F. Bartlett
      Pages 223-242
    4. Victor Nurcombe, Nigel G. Wreford, John F. Bertram
      Pages 251-268
  6. Back Matter
    Pages 269-274

About this book

Introduction

Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade.
Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system.

Editors and affiliations

  • Robert A. Rush
    • 1
  1. 1.Department of Human PhysiologyFlinders UniversityAdelaideAustralia

Bibliographic information

  • DOI https://doi.org/10.1385/1592590608
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-699-4
  • Online ISBN 978-1-59259-060-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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