Abstract
Duplex formation between an oligonucleotide and a strand of mRNA can effectively inhibit the expression of a specific gene via interfering with the cellular protein synthesis process. The antisense technology (1) is regarded as a powerful tool in molecular biology. Advances in the field have also led to the development of many pharmaceutical/therapeutic applications, with a number of oligonucleotides undergoing human clinical trials at present (2). Making the best use of this approach requires a good understanding of the subject, careful planning, a knowledge of limitations and pitfalls, and a capacity to go beyond the basic antisense techniques.
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Epa, W.R., Barrett, G.L., Bartlett, P.F. (2001). Oligonucleotides as Inhibitors of Protein Synthesis. In: Rush, R.A. (eds) Neurotrophin Protocols. Methods in Molecular Biology™, vol 169. Humana Press. https://doi.org/10.1385/1-59259-060-8:223
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DOI: https://doi.org/10.1385/1-59259-060-8:223
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