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Monosaccharide Composition of Mucins

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Glycoprotein Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 125))

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Abstract

Mucin oligosaccharides are constructed by monosaccharide addition to form common cores. This architecture limits the number of constituent monosaccharides. Monosaccharides commonly found in mucins may be divided into neutral (galactose [Gal]; fucose [Fuc]), hexosamines (N-acetylgalactosamine [GalNAc]; N-acetyl-glucosamine [GlcNAc]), and acidic compounds (sialic acids [NeuAc]). Additive heterogeneity comes from the possible substitution with aglycone residues such as sulfate, phosphate, or acetate groups. Prior to their analysis, monosaccharides must be released from the oligosaccharide chain by acidic hydrolysis. Monosaccharide composition can also be achieved on free oligosaccharide-alditols released from the native glycoprotein by reductive alkaline treatment (β-elimination). In this case, GalNAc is converted into N-acetylgalactosaminitol (GalNAc-ol). Different methods are available for the analysis of monosaccharides depending mainly on the amount of material available. Several techniques, such as gas-liquid chromatography (GLC) or high-performance liquid chromatography (HPLC), allow both quantitative and qualitative analysis of monosaccharide mixtures. Other chromatographic or electrophoretic procedures are described herein, but these only allow a rapid qualitative analysis of samples. Single separated monosaccharides may be further identified by physicochemical methods such as mass spectrometry (MS) or nuclear magnetic resonance.

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Authors and Affiliations

  1. Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, UMR CNRS 8576, Lille, France

    Jean-Claude Michalski & Calliope Capon

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  1. Jean-Claude Michalski
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  2. Calliope Capon
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Editors and Affiliations

  1. Bristol Royal Infirmary, Bristol, UK

    Anthony P. Corfield

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© 2000 Humana Press Inc.

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Michalski, JC., Capon, C. (2000). Monosaccharide Composition of Mucins. In: Corfield, A.P. (eds) Glycoprotein Methods and Protocols. Methods in Molecular Biology™, vol 125. Humana Press. https://doi.org/10.1385/1-59259-048-9:159

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  • DOI: https://doi.org/10.1385/1-59259-048-9:159

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-720-5

  • Online ISBN: 978-1-59259-048-3

  • eBook Packages: Springer Protocols

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