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Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems

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Cytochrome P450 Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 107))

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Abstract

A procedure is outlined that is used for purification of heterologously expressed P4503A4 from Escherichia coli membranes. Details of constructron of the particular plasmid expressed (NF14) are presented elsewhere (1, 2). The general procedure can be utilized for other cytochromes P450, with some modification, as described elsewhere (1, 3–7). Several general purification techniques are described in detarl in Chapters 4 and 5 of this volume.

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References

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Vanderbilt University, Nashville, TN

    F. Peter Guengerich, Natalie A. Hosea & Martha V. Martin

Authors
  1. F. Peter Guengerich
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  2. Natalie A. Hosea
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  3. Martha V. Martin
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Queen Mary and Westfie1d College, University of London, UK

    Ian R. Phillips

  2. Department of Biochemistry and Molecular Biology, University College, London, UK

    Elizabeth A. Shephard

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© 1998 Humana Press Inc.

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Peter Guengerich, F., A. Hosea, N., V. Martin, M. (1998). Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems. In: Phillips, I.R., Shephard, E.A. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology™, vol 107. Humana Press. https://doi.org/10.1385/0-89603-519-0:77

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  • DOI: https://doi.org/10.1385/0-89603-519-0:77

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-519-5

  • Online ISBN: 978-1-59259-580-8

  • eBook Packages: Springer Protocols

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