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Yeast Plasmids

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Recombinant Gene Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 62))

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Abstract

The yeast Saccharomyces cerevisiae provides an excellent system to study genes of eukaryotes because it has been extensively characterized genetically and because the molecular mechanisms governing many cellular processes in yeasts are conserved in other organisms. For example, yeasts provide a powerful system for the study of mammalian proteins. However, to study the function of a cDNA encoding a heterologous protein in yeast, the cDNA needs to be cloned in an appropriate vector that permits expression, correct localization, and the posttranslational modification of the product. In this chapter we describe the yeast vectors available for analysis of a new gene and its product and provide two recommended transformation protocols.

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Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA

    Keshav K. Singh

  2. Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand

    Jack A. Heinemann

Authors
  1. Keshav K. Singh
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  2. Jack A. Heinemann
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Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

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© 1997 Humana Press Inc.

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Singh, K.K., Heinemann, J.A. (1997). Yeast Plasmids. In: Tuan, R.S. (eds) Recombinant Gene Expression Protocols. Methods in Molecular Biology, vol 62. Humana Press. https://doi.org/10.1385/0-89603-480-1:113

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  • DOI: https://doi.org/10.1385/0-89603-480-1:113

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-480-8

  • Online ISBN: 978-1-59259-548-8

  • eBook Packages: Springer Protocols

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