Recombinant Gene Expression Protocols

  • Rocky S. Tuan

Part of the Methods in Molecular Biology book series (MIMB, volume 62)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Introduction

    1. Rocky S. Tuan
      Pages 3-8
  3. Expression in E. Coli

    1. Herbert P. Schweizer, RoxAnn R. Karkhoff-Schweizer
      Pages 17-27
    2. Alan C. Christensen
      Pages 29-36
    3. Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén
      Pages 37-54
    4. Daniel G. Yansura, Steven H. Bass
      Pages 55-62
    5. Ophry Pines, Masayori Inouye
      Pages 73-87
    6. Brigitte E. Schoner
      Pages 89-97
  4. Expression in Yeast

    1. Keshav K. Singh, Jack A. Heinemann
      Pages 113-130
    2. Lawrence M. Mylin, James E. Hopper
      Pages 131-148
    3. Virginia L. Price
      Pages 149-157
    4. Ian R. Graham, Alistair Chambers
      Pages 159-169
  5. Viral Expression Systems

    1. Christopher C. Broder, Patricia L. Earl
      Pages 173-197
    2. Kenine E. Comstock, Nathaniel F. Watson, John C. Olsen
      Pages 207-222
    3. Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu et al.
      Pages 223-232
  6. Expression in Nonmammalian Higher Eukaryotes

    1. David R. O’Reilly
      Pages 235-246

About this book

Introduction

The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse­ quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex­ press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap­ ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmammalian eukaryotes such as plants, Xenopus, and insects, as well as in transgenic organisms. In-depth information is presented on the important characteristics of expression cloning vectors and the various methods for efficiently introducing expression constructs into target cells and/ or organisms. Throughout Recombinant Gene Expression Protocols, the authors have consistently striven for a balanced presentation of both background informa­ tion and actual laboratory details.

Editors and affiliations

  • Rocky S. Tuan
    • 1
  1. 1.Thomas Jefferson UniversityPhiladelphia

Bibliographic information

  • DOI https://doi.org/10.1385/0896034801
  • Copyright Information Humana Press 1997
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-480-8
  • Online ISBN 978-1-59259-548-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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