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Electroporation of Mycobacteria

  • Protocol
Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 101))

Abstract

The study of mycobacterial genomes has exploded during the last 10 yr. Initially, no systems were available for the du-ect manipulation of mycobacterial genes in mycobactena, so Escherichia coli was used as the primary cloning host. Several genomic libraries were created (1–5) in E. coli. Although these proved useful for the ldentification of many protein antigens (6), the use of E coli as a cloning host has several limitations. It is now known that many mycobacterial promoters do not function at all in E. coli; therefore, it is difficult to study the expression and control of mycobacterial genes in such a host. In addition, certain posttranslational modifications of proteins do not take place in E. coli and therefore the antigenicity and properties of proteins expressed in E coli may differ (7–9).

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Author information

Authors and Affiliations

  1. Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK

    Tanya Parish

  2. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK

    Neil G. Stoker

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  1. Tanya Parish
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  2. Neil G. Stoker
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Editors and Affiliations

  1. London School of Hygiene and Tropical Medicine, London, UK

    Tanya Parish  & Neil G. Stoker  & 

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© 1998 Humana Press Inc., Totowa, NJ

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Parish, T., Stoker, N.G. (1998). Electroporation of Mycobacteria. In: Parish, T., Stoker, N.G. (eds) Mycobacteria Protocols. Methods in Molecular Biology™, vol 101. Humana Press. https://doi.org/10.1385/0-89603-471-2:129

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  • DOI: https://doi.org/10.1385/0-89603-471-2:129

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-471-6

  • Online ISBN: 978-1-59259-576-1

  • eBook Packages: Springer Protocols

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