Mycobacteria Protocols

  • Tanya Parish
  • Neil G. Stoker

Part of the Methods in Molecular Biology™ book series (MIMB, volume 101)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Tanya Parish, Neil G. Stoker
    Pages 1-13
  3. Bryan W. Allen
    Pages 15-30
  4. John T. Belisle, Michael G. Sonnenberg
    Pages 31-44
  5. Bonnie B. Plikaytis, C. Harold King
    Pages 45-50
  6. Wolfgang J. Philipp, Stephen Gordon, Amalio Telenti, Stewart T. Cole
    Pages 51-63
  7. Eshwar Mahenthiralingam
    Pages 65-75
  8. Tanya Parish, Paul R. Wheeler
    Pages 77-89
  9. Staffan Bergh, Stewart T. Cole
    Pages 109-118
  10. Julie-Ann Gavigan, Carlos Martín
    Pages 119-128
  11. Tanya Parish, Neil G. Stoker
    Pages 129-144
  12. Gary J. Sarkis, Graham F. Hatfull
    Pages 145-173
  13. Lucy A. Brooks
    Pages 175-186
  14. Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giquel et al.
    Pages 187-198
  15. Britt-Inger Marklund, Richard W. Stokes
    Pages 217-224
  16. Elizabeth Norman
    Pages 225-233
  17. Stephen Gordon, Gavin Chung, Peter Andrew
    Pages 235-243

About this book


In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

Mycobacteria Protocols contains a unique collection of both established and recently developed techniques that have been optimized and adapted for use with mycobacteria. Offering an eminently practical introduction for all those carrying out basic or applied research in this important class of bacteria, Mycobacteria Protocols is certain to become the new standard reference in the field, one from which even expert researchers will learn many time-saving techniques.

Editors and affiliations

  • Tanya Parish
    • 1
  • Neil G. Stoker
    • 1
  1. 1.London School of Hygiene and Tropical MedicineLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-471-6
  • Online ISBN 978-1-59259-576-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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