Abstract
Immunofluorescent staining techniques can be used to probe the DNA structure of chromosomes. The primary requirement IS a sequence- or structure-specific antrbody. Most DNA is not immunogeinc, although DNA-binding antibodies are produced spontaneously in the autoimmune drsease systemrc lupus erythematosus (1). In general, these antibodies exhtbtt little sequence or structural specificity and, therefore, are not useful for probing chromosomes except perhaps as controls.
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© 1997 Humana Press Inc., Totowa, NJ
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Lee, J.S., Latimer, L.J.P., Burkholder, G.D. (1997). Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies. In: Fox, K.R. (eds) Drug-DNA Interaction Protocols. Methods in Molecular Biology™, vol 90. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-447-X:189
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DOI: https://doi.org/10.1385/0-89603-447-X:189
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-447-1
Online ISBN: 978-1-59259-574-7
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