Drug'DNA Interaction Protocols

1. Front Matter

   Pages i-x

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2. DNase I Footprinting

   • Keith R. Fox

   Pages 1-22

3. Quantitative DNA Footprinting

   • James C. Dabrowiak, Jerry Goodisman, Brian Ward

   Pages 23-42

4. Uranyl Photoprobing of DNA Structures and Drug–DNA Complexes

   • Niels Erik Møllegaard, Peter E. Nielsen

   Pages 43-49

5. Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro

   • Christian Bailly, Michael J. Waring

   Pages 51-79

6. Footprinting Studies with Nucleosome-Bound DNA

   • Philip M. Brown, Keith R. Fox

   Pages 81-93

7. A Gel Mobility Shift Assay for Probing the Effect of Drug–DNA Adducts on DNA-Binding Proteins

   • Suzanne M. Cutts, Andrew Masta, Con Panousis, Peter G. Parsons, Richard A. Sturm, Don R. Phillips

   Pages 95-106

8. An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug–Binding Sites

   • Suzanne M. Cutts, Con Panousis, Andrew Masta, Don R. Phillips

   Pages 107-115

9. DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug–DNA Interactions

   • Thomas P. Dooley, Katherine L. Weiland

   Pages 117-125

10. Transcriptional Footprinting of Drug–DNA Interactions

    • Don R. Phillips, Carleen Cullinane

    Pages 127-145

11. Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays

    • John A. Hartley, Michael D. Wyatt

    Pages 147-156

12. PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells

    • Keith A. Grimaldi, John A. Hartley

    Pages 157-180

13. Analysis of DNA-Binding Antibodies

    • Jeremy S. Lee, Laura J. P. Latimer, Jamshid Tanha

    Pages 181-188

14. Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies

    • Jeremy S. Lee, Laura J. P. Latimer, Gary D. Burkholder

    Pages 189-193

15. Optical Absorbance and Fluorescence Techniques for Measuring DNA–Drug Interactions

    • Terence C. Jenkins

    Pages 195-218

16. Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves

    • W. David Wilson, Farial A. Tanious, Maria Fernandez-Saiz, C. Ted Rigl

    Pages 219-240

17. Electric Dichroism

    • Dietmar Porschke

    Pages 241-258

18. Calorimetric Techniques for Studying Drug–DNA Interactions

    • Harry P. Hopkins Jr.

    Pages 259-268

19. Methods for the Studies of Drug Dissociation from DNA

    • Fu-Ming Chen

    Pages 269-274

20. Back Matter

    Pages 275-278

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In Drug-DNA Interactions, expert researchers describe powerful molecular techniques designed to illuminate and explore the interaction of drugs and ligands with DNA, often perfected in their own laboratories, . These easily reproducible methods involve sequence recognition properties, as well as physical approaches to measuring both the strength of interaction and the mode of drug binding to DNA. Drug interactions are also examined from a cellular perspective and for their usefulness in the development of new therapeutic agents.

Drug-DNA Interactions will be highly useful to all investigators needing the kind of detailed experimental and technical information often omitted from scientific papers. Those wishing to expand their repertoire of experimental techniques or to improve their current techniques will also find these powerful methods particularly helpful in elucidating the action of existing therapeutic agents, as well as in designing new anticancer drugs with improved action and reduced toxicity.

"Methods in Molecular Biology is a long standing, respected series that provides protocols for the researcher. ..this particular book is a welcome addition for the researcher. It is recommended for all medical and science libraries."-Reviewer: H. Robert Malinowsky, E-STREAMS

• University of Southampton, UK

  Keith R. Fox

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