Skip to main content
SpringerLink
Log in

Methods of Culturing HIV-1 from Semen

  • Protocol
HIV Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 17))

  • 2157 Accesses

Abstract

The predominant mode of transmission of HIV-1 worldwide is sexual intercourse. Therefore, there has been growing interest in studying HIV-1 in genital secretions. Given the urgency to develop a vaccine to protect against HIV-1, techniques have been developed to isolate and quantitate HIV-1 from genital secretions.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Vernazza, P. L., Eron, J. J., and Fiscus, S.A. (1996) Sensitive method for the detection of infectious HIV in semen of seropositive individuals. J. Virol. Methods 56, 33–40.

    Article  PubMed  CAS  Google Scholar 

  2. Van Voorhis, B., Martinez, A., Mayer, K., and Anderson, D. J. (1991) Detection of human immunodeficiency virus type 1 in semen from seropositive men using culture and polymerase chain reaction deoxyribonucleic acid amplification techniques. Fertil. Steril. 55, 588–594.

    PubMed  Google Scholar 

  3. Mermin, J. H., Holodniy, M., Katzenstein, D. A., and Merigan, T. C. (1991) Detection of human immunodeficiency virus DNA and RNA in semen by the polymerase chain reaction. JID 164, 769–772.

    PubMed  CAS  Google Scholar 

  4. Dyer, J., Gilliam, B. L., Eron, J. J. Jr., Grosso, L., Cohen, M. S., and Fiscus, S. (1996) Quantitation of human immunodeficiency virus type 1 RNA in cell-free seminal plasma: comparison of NASBA and amplicor reverse transcription PCR amplification. J. Virol. Methods 60, 161–170.

    Article  PubMed  CAS  Google Scholar 

  5. Vernazza, P. L., Eron, J. J. Jr., Cohen, M. S., van der Horst, C. M., Troiani, L., and Fiscus, S. A. (1994) Detection and biologic characterization of infectious HIV-1 in semen of seropositive men. AIDS 8, 1325–1329.

    Article  PubMed  CAS  Google Scholar 

  6. Krieger, J. N., Coombs, R. W., Collier, A. C., Koehler, J. K., Ross, S. O., Chaloupka, K., et al. (1991) Fertility parameters in men infected with human immunodeficiency virus. JID 164, 464–469.

    PubMed  CAS  Google Scholar 

  7. Krieger, J. N., Coombs, R. W., Collier, A. C, Ross, S. O., Speck, C., Corey, L. (1995) Seminal shedding of human immunodeficiency virus type 1 and human cytomegalovirus: evidence for different immunologic controls. JID 171, 1018–1022.

    PubMed  CAS  Google Scholar 

  8. Krieger, J. N., Coombs, R. W., Colllier, A. C., Ross, S. 0., Zeh, J. E., et al. (1995) Intermittent shedding of human immunodeficiency virus in semen: implications for sexual transmission. J. Urol. 154, 1035–1040.

    Article  PubMed  CAS  Google Scholar 

  9. Anderson, D. J., O’Brien, T. R., Politch, J. A., Martinez, A., Seage, G. R. III, Padian N., et al. (1992) Effects of disease stage and zidovudine therapy on the detection of human immunodeficiency virus type 1 in semen. JAMA 267, 2769–2774.

    Article  PubMed  CAS  Google Scholar 

  10. Gilliam, B. L., Dyer, J., Fiscus, S. A., Marcus, C., Zhou, S., Wathen, L., Freimuth, W. W., Cohen, M. S., Eron, J. J. Jr. (1997) Effects of reverse transcriptase inhibitor therapy on HIV-1 viral burden in semen. J. Acquir. Immun. Defic. Syndr. Hum. Retrovirol. 15, 54–60.

    CAS  Google Scholar 

  11. Myers, L. E., McQuay, L. J., and Hollinger, F. B. (1994) Dilution assay statistics. J. Clin. Microbiol. 32, 732–739.

    PubMed  CAS  Google Scholar 

  12. Wolff, H., and Anderson, D. J., (1988) Immunohistologic characterization and quantitation of leukocyte subpopulations in human semen. Fertil. Steril. 49, 497–504.

    PubMed  CAS  Google Scholar 

  13. Vernazza, P. L., Gilliam, B. L., Dyer, J., Fiscus, S. A., Eron, J. J., Frank, A. C, and Cohen, M. S. (1997) Quantitation of HIV in semen: Correlation with antiviral treatment and immune status. AIDS 11, 987–993.

    Article  PubMed  CAS  Google Scholar 

  14. Vernazza, P. L., Gilliam, B. L., Flepp, M., Dyer, J. R., Frank, A. C., Fiscus, S. A., et al. (1997) Effect of antiviral treatment on shedding of HIV-1 in semen. AIDS 11, 1249–1254.

    Article  PubMed  CAS  Google Scholar 

  15. Krieger, J. N., Coombs, R. W., Collier, A. C, Ross, S. O., Chaloupka, K., Cummings, D. K., et al. (1991) Recovery of human immunodeficiency virus type 1 from semen: minimal impact of stage of infection and current antiviral chemotherapy. JID 163, 386–388.

    PubMed  CAS  Google Scholar 

  16. Cooms, R. W., Speck, C. E., Hughes, J. P., Lee, W., Sampoleo, R., Ross, S. O., et al. (1998) Association between culturable human immunodeficiency virus type 1 (HIV-1) in semen and HIV-1 RNA levels in semen and blood: evidence for compartmentalization of HIV-1 between semen and blood. JID 111, 320–330.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Henry M. Jackson Foundation, Rockville, MD

    Bruce L. Gilliam

Authors
  1. Bruce L. Gilliam
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Rockville, MD

    Nelson L. Michael MD, PhD

  2. Henry M. Jackson Foundation, Rockville, MD

    Jerome H. Kim MD

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Gilliam, B.L. (1999). Methods of Culturing HIV-1 from Semen. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:51

Download citation

  • DOI: https://doi.org/10.1385/0-89603-369-4:51

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-369-6

  • Online ISBN: 978-1-59259-601-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation