Overview
- Editors:
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Nelson L. Michael
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Walter Reed Army Institute of Research, Rockville
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Jerome H. Kim
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Henry M. Jackson Foundation, Rockville
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Table of contents (43 protocols)
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Front Matter
Pages i-xiii
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Virology
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- Mark K. Louder, John R. Mascola
Pages 23-27
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- Mark K. Louder, John R. Mascola
Pages 29-33
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- Rong Liu, Benjamin Chen, Nathaniel R. Landau
Pages 35-40
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- Aimee L. Edinger, Robert W. Doms
Pages 41-49
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Molecular Biology
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- Richard A. McDonald, Christopher A. D. Smith
Pages 71-81
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- Wei Dong, Anindita Kar Roy, Yen Li
Pages 83-87
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- Maryanne Vahey, Sandra C. Barrick, Martin Nau
Pages 99-118
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- Davide Zella, Paola Secchiero
Pages 119-124
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- Sharon A. Cassol, Stanley Read, Bruce G. Weniger, Richard Pilon, Barbara Leung, Theresa Mo
Pages 125-138
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- Sharon A. Cassol, Francisco Diaz-Mitoma, Richard Pilon, Michelle Janes, D. William Cameron
Pages 139-149
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- Omar Bagasra, Lisa E. Bobroski, Mohammad Amjad, Matthew Memoli, Maureen V. Abbey
Pages 165-183
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- Loretta Tse, Nelson L. Michael
Pages 185-196
About this book
The worldwide impact of infection with human immunodeficiency virus type 1 (HIV- is reflected in the cumulative number ofHIV- 1 infections, which is now predicted to exceed 40 million by the year 2000---equivalent to the n- ber of humans who perished in World War II. The medical and scientific - sponse to the HIV-1 pandemic has steadily grown since its recognition in 1981. The outlay by the United States alone for HIV research funded by the National Institutes of Health in 1997 was $1. 4 billion. Laboratory-based HIV research has brought together academic clinicians, retrovirologists, molecular biologists, and immunologists in the formation of research teams attempting to dissect the viral and host factors contributing to disease pathogenesis. Increasing focus is being placed on those aspects of viral biology and host immune responses that bear on the development of vaccines to prevent HIV infection. HIVProtocols reflects the state of HIV research in several ways. First, chapters are organized into four sections: Virology, Molecular Biology, Humoral Immunology, and Cellular Immunology. This organization is a natural consequence of the diverse scientific disciplines that have been attracted to HIV research. Second, the chapters reflect such diverse research directions as viral coreceptor usage, quantitation of viral genomes, HIV promoter function, B-cell epitope mapping, and measurements of T-cell function, each of which bears on the goal of understanding the viral and host immune responses that will be critical to the design of effective preventive vaccines.
Reviews
". . . a great companion to the more general laboratory protocol manuals. . . provide[s] an impressively up-to-date collection of laboratory methods for every common and not-so-common AIDS lab experiment."-Antiviral Research
HIV Protocols is a good addition to a basic science laboratory that processes various HIV clinical specimens. Graduate students or clinical fellows starting out on their careers in basic experimental science will find it most useful....Overall, this book compiles a number of general basic science protocols in the fields of virology, molecular biology and immunology as applied to the study of HIV. The thorough nature of the materials and methods section of each chapter ensures that the reader will get off to a good start when using these techniques in their own laboratory." -Can J Infect Dis
Editors and Affiliations
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Walter Reed Army Institute of Research, Rockville
Nelson L. Michael
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Henry M. Jackson Foundation, Rockville
Jerome H. Kim
