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Quantification of HIV-1 RNA in Dried Plasma Spots (DPS)

A Field Approach to Therapeutic Monitoring

  • Protocol
HIV Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 17))

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Abstract

The ability to accurately measure viral RNA in the plasma (1–3) and intracellular (4–7) compartments of HIV-1-infected persons has led to a dramatic improvement in the understanding of the natural history of HIV-1 and AIDS. A number of recent studies have convincingly demonstrated that high levels of viral replication occur at all stages of disease (8–10), and that changes in viral RNA load are predictive of disease outcome (11,12), and response to therapy (13,14). These findings, combined with the introduction of potent new antivirals (15,16), have stimulated a growing interest in viral load monitoring, both as a function of disease status, and as a predictor of disease progression and therapeutic efficacy.

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Author information

Authors and Affiliations

  1. Ottawa General Hospital Research Institute, Ottawa, Ontario, Canada

    Sharon A. Cassol, Richard Pilon & Michelle Janes

  2. Department of Microbiology and Immunology, University of Ottawa, Ottawa, Canada

    Francisco Diaz-Mitoma

  3. Department of Laboratory Medicine, Children’s Hospital of Eastern Ontario, Ottawa, Ontario, Canada

    D. William Cameron

Authors
  1. Sharon A. Cassol
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  2. Francisco Diaz-Mitoma
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  3. Richard Pilon
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  4. Michelle Janes
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  5. D. William Cameron
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Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Rockville, MD

    Nelson L. Michael MD, PhD

  2. Henry M. Jackson Foundation, Rockville, MD

    Jerome H. Kim MD

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© 1999 Humana Press Inc., Totowa, NJ

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Cassol, S.A., Diaz-Mitoma, F., Pilon, R., Janes, M., Cameron, D.W. (1999). Quantification of HIV-1 RNA in Dried Plasma Spots (DPS). In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:139

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  • DOI: https://doi.org/10.1385/0-89603-369-4:139

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-369-6

  • Online ISBN: 978-1-59259-601-0

  • eBook Packages: Springer Protocols

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