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Electroblotting of Proteins from Polyacrylamide Gels

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Protein Purification Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 59))

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Abstract

The ability of polyacrylamide gel electrophoresis (PAGE) techniques to resolve individual proteins of complex mixtures has resulted in this group of methods being indispensable to the protein biochemist. Although PAGE procedures can provide characterization of proteins in terms of their charge, size, relative hydrophobicity, and abundance, they provide no direct clues to the identity or function of the separated components.

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© 1996 Humana Press Inc.

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Dunn, M.J. (1996). Electroblotting of Proteins from Polyacrylamide Gels. In: Doonan, S. (eds) Protein Purification Protocols. Methods in Molecular Biology™, vol 59. Humana Press. https://doi.org/10.1385/0-89603-336-8:363

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  • DOI: https://doi.org/10.1385/0-89603-336-8:363

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-336-8

  • Online ISBN: 978-1-59259-545-7

  • eBook Packages: Springer Protocols

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