Protein Purification Protocols

  • Shawn Doonan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 59)

Table of contents

  1. Front Matter
    Pages i-x
  2. Shawn Doonan
    Pages 1-16
  3. Peter R. Shewry, Roger J. Fido
    Pages 23-29
  4. D. Margaret Worrall
    Pages 31-37
  5. Paul Bridge
    Pages 39-48
  6. Alyson K. Tobin
    Pages 57-68
  7. Robert J. Beynon, Simon Oliver
    Pages 81-93
  8. Shawn Doonan
    Pages 95-102
  9. Shawn Doonan
    Pages 103-113
  10. David Sheehan, Richard FitzGerald
    Pages 145-150
  11. Paul O’Farrell
    Pages 151-155
  12. Paul Cutler
    Pages 157-168
  13. D. Margaret Worrall
    Pages 169-176
  14. Iris West, Owen Goldring
    Pages 177-186
  15. George W. Jack, David J. Beer
    Pages 187-196

About this book

Introduction

Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro­ teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech­ nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato­ ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom­ ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.

Editors and affiliations

  • Shawn Doonan
    • 1
  1. 1.Department of Life SciencesUniversity of East LondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033368
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-336-8
  • Online ISBN 978-1-59259-545-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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