Abstract
Membrane ultrafiltration (UF) is a pressure-modified, convective process that uses semipermeable membranes to separate species in aqueous solutions by molecular size, shape, and/or charge. It separates solvents from solutes (i.e., the dissolved species) of various sizes. The result of removing solvent from a solution is solute concentration or enrichment. Repeated or continuous dilution and reconcentration are used to remove salts or exchange solvent (in such applications as buffer exchange). Definitions of some terms used in UF are given in Table 1.
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© 1996 Humana Press Inc.
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Schratter, P. (1996). Purification and Concentration by Ultrafiltration. In: Doonan, S. (eds) Protein Purification Protocols. Methods in Molecular Biology™, vol 59. Humana Press. https://doi.org/10.1385/0-89603-336-8:115
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DOI: https://doi.org/10.1385/0-89603-336-8:115
Publisher Name: Humana Press
Print ISBN: 978-0-89603-336-8
Online ISBN: 978-1-59259-545-7
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