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Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells

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Human Cell Culture Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 2))

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Abstract

More than 500 different mutations have been described to date on the cystic fibrosis (CF) gene (1). The most frequent mutation is the so-called ΔF508 mutation, which accounts for 30–50%;of CF chromosomes in southern European countries and for 50–80%; of CF chromosomes in the United States, Canada, Argentina, and central and northern European countries

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References

  1. Cystzc Fzbrosu mutatzon data Prrvrleged commumcation prepared for members of the CF Genetic Analysts Consortmm, April 1995

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  2. Fanen, P., Ghanem, N., Vidaud, M., Besmond, C., Martin, J., Costes, B., Plassa, F., and Goossens, M. (1992) Molecular charactertzation of cystic fibrosis 16 novel mutations identified by analysis of the whole cystic fibrosis conductance transmembrane regulator (CFTR) coding regions and splice site junctions Genomics 13, 770–776

    Article  PubMed  CAS  Google Scholar 

  3. Ghanem, N., Fanen, P., Martin, J., Conteville, P., Yahia-Cherif, Z., Vidaud, M., and Goossens, M. (1992) Exhaustive screening of exon 10 CFTR gene mutations and polymorphisms by denaturing gradient gel electrophoresis* applications to genetic counselling in cystic fibrosis Mol. Cell. Probes. 6, 27–31

    Article  PubMed  CAS  Google Scholar 

  4. Costes, B., Girodon, E., Ghanem, M., Chassrgnol, M., Thuong, N. T., Dupret, D., and Goossens, M. (1993) Psoralen-modified oligonucleotrde primers improve detection of mutations by denaturing gradient gel electrophoresis and provide an alternative to GC-clamping Hum. Mol. Genet. 2, 393–397

    Article  PubMed  CAS  Google Scholar 

  5. Vidaut, M., Fanen, P., Martin, J., Ghanem, M., Nrcoles, S., and Goossens, M. (1990) 3 point mutations in the CFTR gene in french cystic patients. identification by denaturing gradient gel electrophoresis Hum Genet 85, 445–449

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© 1996 Humana Press Inc., Totowa, NJ

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Vega, M.A. (1996). Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:193

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  • DOI: https://doi.org/10.1385/0-89603-335-X:193

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-335-1

  • Online ISBN: 978-1-59259-586-0

  • eBook Packages: Springer Protocols

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