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  • Book
  • © 1996

Human Cell Culture Protocols

Editors:

  1. Gareth E. Jones

    1. King’s College, University of London, UK

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 2)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  3. Cultivation of Normal Human Epidermal Melanocytes

    • Mei-Yu Hsu, Meenhard Herlyn
    Pages 9-20

  6. Growth and Differentiation of Human Adipose Stromal Cells in Culture

    • Brenda Strutt, Wahid Khalil, Donald Killinger
    Pages 41-51

  7. Human Fetal Brain Cell Culture

    • Mark P. Mattson
    Pages 53-62

  9. Cell Culture of Human Brain Tumors on Extracellular Matrices

    • Manfred Westphal, Hildegard Nausch, Dorothea Zirkel
    Pages 81-99

  11. Human Thymic Epithelial Cell Cultures

    • Anne H. M. Galy
    Pages 111-119

  15. Purification of Peripheral Blood Natural Killer Cells

    • Ian M. Bennett, Bice Perussia
    Pages 161-171

  16. Cystic Fibrosis Airway Epithelial Cell Culture

    • Manuel A. Lega
    Pages 173-183

  19. Human Tracheal Gland Cells in Primary Culture

    • Marc D Merten
    Pages 201-216
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About this book

Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more. The protocols eliminate much of the chore of adapting techniques initially developed for animal cell culture systems or the time spent in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. They allow researchers to use successfully high quality in vitro cultures as models to explore the disease process.
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Reviews

This book is for the clinical scientist beginning to use in vitro systems and for anyone making the transition from animal cell culture to the use of human tissue and cell lines.- Journal of Toxicology: Clinical Toxicology
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Editors and Affiliations

  • King’s College, University of London, UK

    Gareth E. Jones

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Bibliographic Information

  • Book Title: Human Cell Culture Protocols

  • Editors: Gareth E. Jones

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/089603335X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1996

  • eBook ISBN: 978-1-59259-586-0Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 545

  • Number of Illustrations: 101 b/w illustrations

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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eBook USD 79.99
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  • Own it forever

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