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The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Complex

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Species Diagnostics Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 50))

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Abstract

Mosquitoes of the Anopheles gambiae complex include the major vectors of malaria in tropical Africa. Six different sibling species within the complex have been identified on the basis of mating incompatibility (13). None of them can be distinguished using morphological characteristics. An. gambiae sensu stricto and An. arabiensis are the two most efficient vectors within the complex and are widespread throughout tropical Africa being sympatric over much of their range. An. merus and An. melas, two saltwater-associated forms, are vectors in coastal regions of East and West Africa, respectively, whereas An. bwambae is a vector confined to the Semliki forest in Uganda. An. quadriannulatus is mainly zoophilic and therefore not considered to be a vector of malaria; it is found in limited locations in East and South Africa and Ethiopia.

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References

  1. Davidson, G. (1964) Anopheles gambiae, a complex of species. Bull. World Health Organisation 31, 625–634.

    CAS  Google Scholar 

  2. Davidson, G. and Hunt, R. H. (1973) The crossing and chromosome characteristics of a new, sixth species in the Anopheles gambiae complex. Parassitologia 15, 121–128.

    PubMed  CAS  Google Scholar 

  3. Davidson, G., Paterson, H. E., Coluzzi, M., Mason, G. F., and Micks, D. W. (1967) The Anopheles gambiae complex, in Genetics of Insect Vectors of Disease (Wright, J. W. and Pal, R., eds.), Elsevier, Amsterdam, pp. 211–250.

    Google Scholar 

  4. Service, M. W. (1982) Importance of vector ecology in vector disease control in Africa. Bull. Soc. Vector Ecol. 7, 1–13.

    Google Scholar 

  5. Coluzzi, M., and Sabatini, A. (1967) Cytogenetic observations on species A and B of the Anopheles gambiae complex. Parassitologia 9, 73–88.

    Google Scholar 

  6. Coluzzi, M., and Sabatini, A. (1968) Cytogenetic observations on species C of the Anopheles gambiae complex. Parassitologia 10, 155–165.

    Google Scholar 

  7. Coluzzi, M., and Sabatini, A. (1969) Cytogenetic observations on the saltwater species Anopheles merus and Anopheles melas of the Anopheles gambiae complex. Parassitologia 11, 177–187.

    Google Scholar 

  8. Green, C. A. (1972) Cytological maps for the identification of females of the three freshwater species of the Anopheles gambiae complex. Ann. Trop. Med. Parasitol. 66, 143–147.

    PubMed  CAS  Google Scholar 

  9. Mahon, R. J., Green, C. A., and Hunt, R. H. (1976) Diagnostic allozymes for the routine identification of adults of the Anopheles gambiae complex (Diptera, Culicidae). Bull. Entomol. Res. 66, 25–31.

    Article  CAS  Google Scholar 

  10. Miles, S. J. (1978) Enzyme variation in the Anopheles gambiae Giles group of species (Diptera: culicidae). Bull. Entomol. Res. 68, 85–96.

    Article  CAS  Google Scholar 

  11. Miles, S. J. (1979) The Anopheles gambiae complex: a biochemical key. J. Med. Entomol. 15, 297–299.

    PubMed  CAS  Google Scholar 

  12. Carlson, D. A., and Service, M. W. (1979) Differentiation between species of the Anopheles gambiae complex (Diptera: culicidae) by analysis of cuticular hydrocarbons. Annal. Trop. Med. Parasitol. 73, 589–592.

    CAS  Google Scholar 

  13. Hamilton, R. J., and Service, M. W. (1983) Value of cuticular and internal hydrocarbons for the identification of larvae of Anopheles gambiae Giles, Anopheles arabiensis Patton and Anopheles melas Theobald. Annal. Trop. Med. Parasitol. 77, 203–210.

    CAS  Google Scholar 

  14. Paskewitz, S. M. and Collins, F. H. (1990) Use of polymerase chain reaction to identify mosquito species of the Anopheles gambiae complex. Med. Vet. Entomol. 4, 367–373.

    Article  PubMed  CAS  Google Scholar 

  15. Paskewitz, S. M., Ng, K., Coetzee, M., and Hunt, R. H. (1993) Evaluation of the polymerase chain reaction method for identifying members of the Anopheles gambiae (Diptera: Culicidae) complex in South Africa. J. Med. Entomol. 30, 953–957.

    PubMed  CAS  Google Scholar 

  16. Gale, K. R., and Crampton, J. M. (1987) DNA probes for species identification of mosquitoes in the Anopheles gambiae complex. Med. Vet. Ent. 1, 127–136.

    Article  CAS  Google Scholar 

  17. Gale, K. R. and Crampton, J. M. (1987) A DNA probe to distinguish the species Anopheles quadriannulatus from other species of the Anopheles gambiae complex. Trans. Roy. Soc. Trop. Med. Hyg. 81, 842–846.

    Article  PubMed  CAS  Google Scholar 

  18. Gale, K. R., and Crampton, J. M. (1988) Use of a male specific DNA probe to distinguish female mosquitoes of the Anopheles gambiae complex. Med. Vet. Ent. 2, 77–79.

    Article  CAS  Google Scholar 

  19. Hill, S. M. and Crampton, J. M. (1994) Synthetic DNA probes to identify members of the Anopheles gambiae complex and to distuiguish the two major vectors of malaria within the complex, An. gambiae ss and An. arabiensis. Am. J. Trop. Med. Hyg. 50, 312–321.

    PubMed  CAS  Google Scholar 

  20. Hill, S. M., Urwin, R., Knapp, T. F., and Crampton, J. M. (1991) Synthetic DNA probes for the identification of sibling species within the Anopheles gambiae complex. Med. Vet. Entomol. 5, 455–463.

    Article  PubMed  CAS  Google Scholar 

  21. Hill, S. M., Urwin, R., and Crampton, J. M. (1991) A comparison of non-radioactive labeling and detection systems with synthetic oligonucleotide probes for the species identification of mosquitoes in the Anopheles gambiae complex. Am. J. Trop. Med. Hyg. 44, 609–622.

    PubMed  CAS  Google Scholar 

  22. Hill, S. M., Urwin, R., and Crampton, J. M. (1992) A simplified, non-radioactive DNA probe protocol for the field identification of insect vector specimens. Trans. Roy. Soc. Trop. Med. Hyg. 86, 213–215.

    Article  PubMed  CAS  Google Scholar 

  23. Panyim, S., Yasothornsrikul, S., Tungpradubkul, S., Baimai, V., Rosenberg, R., Andre, R. G., and Green, C. A. (1988) Identification of isomorphic malaria vectors using a DNA probe. Am. J. Trop. Med. Hyg. 38, 47–49.

    PubMed  CAS  Google Scholar 

  24. Booth, D. R., Mahon, R. J., and Sriprakash, K. S. (1991) DNA probes to identify the members of the Anopheles farauti complex. Med. Vet. Entomol. 5, 447–454.

    Article  PubMed  CAS  Google Scholar 

  25. Hartas, J., Whelan, P., Sriprakash, K. S., and Booth, D. (1992) Oligonucleotide probes to identify three sibling species of the Anopheles farauti laveran (Diptera: Culicidae) complex. Trans. Roy. Soc. Trop. Med. Hyg. 86, 210–212.

    Article  PubMed  CAS  Google Scholar 

  26. Cockbum, A. F., Tarrant, C. A., and Mitchell, S. (1988) Use of DNA probes to distinguish sibling species of the Anopheles quadrimaculatus complex (contrib). Flo. Entomol. J. Rep. 71, 299–302.

    Article  Google Scholar 

  27. Post, R. J., and Crampton, J. M. (1988) The taxonomic use of a variation in repetitive DNA sequences in the Stmulium damnosum complex, in Biosystematics of Haematophagous Insects (Service, M. W., ed.), Clarendon, Oxford, UK, pp 245–256.

    Google Scholar 

  28. Post, R. J., and Flook, P (1992) DNA probes for the identification of members of the Simulium damnosum complex (Diptera: Simulidae). Med. Vet. Entomol. 6, 379–384.

    Article  PubMed  CAS  Google Scholar 

  29. Ready, P. D., Smith, D. F., and Killick-Kendrick, R. (1988) DNA hybridisations on squash blotted sandflies to detect both Phlebotomus papatasi and infecting Leishmania major. Med. Vet. Entomol. 2, 109–116.

    Article  PubMed  CAS  Google Scholar 

  30. Delves, C. J., Goman, M., Ridley, R. G., Matile, H., Lensen, T. H. W., Ponnudarai, T., and Scaife, J. G. (1989) Identification of Plasmodium falciparum infected mosquitoes using a probe containing repetitive DNA. Mol. Biochem. Parasitol. 32, 105–112.

    Article  PubMed  CAS  Google Scholar 

  31. Dissanayake, S., and Piessens, W. F. (1992) Identification of filarial larvae in vectors by DNA hybridisation Parasitol. Today 8, 67–

    Article  PubMed  CAS  Google Scholar 

  32. Gibson, W. C., Dukes, P., and Gashumba, J. K. (1988) Species-specific DNA probes for identification of African trypanosomes in tsetse flies. Parasitology 97, 63–73.

    Article  PubMed  Google Scholar 

  33. Beier, J. C., Perkins, P. V., Wirtz, R. A., Koros, J., Diggs, D., Gargan, T. P., and Koech, D. K. (1988) Bloodmeal identification by direct enzyme-linked immunosorbent assay (ELISA) tested on iAnopheles (Diptera: Culicidae) in Kenya. J. Med. Entomol. 25, 9–16.

    PubMed  CAS  Google Scholar 

  34. Wirtz, R. A., Burkot, T. R., Graves, P. M., and Andre, R. G. (1987) Field evaluation of enzyme-linked immunosorbent assays (ELISAs) for Plasmodium falciparum and Plasmodium vivax sporozoites in mosquitoes (Diptera: Culicidae) from Papua New Guinea. J. Med. Entomol. 24, 433–437.

    PubMed  CAS  Google Scholar 

  35. Graziosi, C., Sakai, R. K., Romans, P., Miller, L. H., and Wellems, T. E. (1990) Method for in situ hybridization to polytene chromosomes from ovarian nurse cells of Anopheles gambiae. J. Med. Entomol. 27, 905–912.

    PubMed  CAS  Google Scholar 

  36. Tchen, P., Anxolabehere, D., Nouaud, D., and Periquet, G. (1985). Hybridisation on squashed flies: a method to detect gene sequences in individual Drosophila. Anal. Biochem. 150, 415–420.

    Article  PubMed  CAS  Google Scholar 

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© 1996 Humana Press Inc.

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Hill, S.M., Crampton, J.M. (1996). The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Complex. In: Clapp, J.P. (eds) Species Diagnostics Protocols. Methods in Molecular Biology™, vol 50. Humana Press. https://doi.org/10.1385/0-89603-323-6:305

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  • DOI: https://doi.org/10.1385/0-89603-323-6:305

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-323-8

  • Online ISBN: 978-1-59259-537-2

  • eBook Packages: Springer Protocols

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