Abstract
The detection and identification of the four Plasmodium species that infect humans in samples obtained from the human or the insect hosts, are central for all the studies aimed at understanding the biology, immunology, pathology, and epidemiology of the malaria parasite. Knowledge of these areas underpins the instigation and implementation of measures aimed at controlling a disease that has afflicted, and continues to afflict a major portion of humanity with far-reaching social and economic consequences.
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References
Spielman, A., Perrone, J. B., Teklehaimanot, A., Balcha, F., Wardlaw, S. C., and Levine, R. A. (1988) Malaria diagnosis by direct observation of centrifuged samples of blood. Am. J Trop Med. Hyg 39, 337–342.
Kawamoto, F. (1991) Rapid diagnosis of malaria by fluorescence microscopy. Lancet 337, 624, 625.
Beier, J. C., Perkins, P. V., Wirtz, R. A., Whitmire, R. E., Mugambi, M., and Hockmeyer, W. T. (1987) Field evaluation of an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum sporozoite detection in anopheline mosquitoes from Kenya. Am. J Trop Med. Hyg 36, 459–468.
Taylor, D. W. and Voller, A. (1993) The development and validation of a simple antigen detection ELISA for Plasmodium falciparum malaria. Trans R Sot Trop Med. Hyg 87, 29–31.
Shiff, C. J., Premji, Z., and Minjas, J. N. (1993) The raped manual ParaSight®-F test A new diagnostic tool for Plasmodium falciparum infection Trans R Soc Trap Med. Hyg 87, 646–648.
Barker, R. H. J. (1990) DNA probe diagnosis of parasitic infections. Exp Parasitol 70, 494–499.
Saiki, R. K., Gelfand, D. H., Stoffel, S., Sharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491.
Tirasophon, W., Ponglikitmongkol, M., Wilairat, P., Boonsaeng, V., and Panyim, S. (1991) A novel detection of a single Plasmodium falciparum in infected blood Biochem Biophys Res Commun 175, 179–184.
Barker, R. H. J., Banchongaksorn, T., Courval, J. M., Suwonkerd, W., Rimwungtragoon, K., and Wirth, D. F. (1992) A sample method to detect Plasmodium falciparum directly from blood samples using the polymerase chain reaction. Am J Trap. Med. Hyg 46, 416–426.
Brown, A. E., Kain, K. C, Pipithkul, J., and Webster, H. K. (1992) Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P vivax infections undetected by conventional microscopy. Trans R Soc Trap. Med. Hyg 86, 609–612.
Snounou, G., Viriyakosol, S., Jarra, W., Thaithong, S., and Brown, K. N. (1993) Identification of the four human malaria species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol 58, 283–292.
Snounou, G., Viriyakosol, S., Zhu, X. P., Jarra, W., Pinheiro, L., Rosario, V. E. d., Thaithong, S., and Brown, K. N. (1993) High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61, 315–320.
McCutchan, T. F., Cruz, V. F. D. L., Lal, A., Gunderson, J. H., Elwood, H. J., and Sogin, M. L. (1988) Primary sequence of two small ribosomal RNA genes from Plasmodium falciparum. Mol. Biochem Parasitol 28, 63–68.
Waters, A. P. and McCutchan, T. F. (1989) Rapid, sensitive diagnosis of malaria based on ribosomal RNA. Lancet i, 1343–1346.
Waters, A. P. and McCutchan, T. F. (1989) Partial sequence of the asexually expressed SU rRNA gene of Plasmodium vivax. Nucleic Acids Res 17, 2135
Goman, M., Mons, B, and Scaife, J. (1991) The complete sequence of a Plasmodium malariae SSU rRNA gene and its comparison to other plasmodial SSU rRNA genes MOl Biochem Parasitol 45, 281–288.
Kain, K. C. and Lanar, D. E. (1991) Determination of genetic variation within Plasmodium falciparum by enzymatically amplified DNA from filter paper disks impregnated with whole blood. J. Clin Microbiol 29, 1171–1174.
Snounou, G., Pinheiro, L., Gonçalves, A., Fonseca, L., Dias, F., Brown, K. N., and Rosario, V. E. d. (1993) The importance of sensitive detection of malaria parasites in the human and mosquito hosts in epidemiological studies, as shown by the analysis of field samples from Guinea Bissau. Trans. R. Soc Trop Med Hyg 87, 64–53.
Ranford-Cartwright, L. C., Balfe, P., Carter, R., and Walliker, D. (1991) Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts. Mol. Biochem Parasitol. 49, 239–244.
Foley, M., Ranford-Cartwright, L. C., and Babiker, H. A. (1992) Rapid and simple method for isolating malaria DNA from finger prick samples of blood. Mol. Biochem Parasitol. 53, 241–244.
Wilson, S. M. (1994) Detection of malaria parasites by PCR. Trans R Soc Trop. Med. Hyg. 88, 363.
Snounou, G., Brown, K. N., and Rosario, V. E. d. (1994) Detection of malaria parasites by PCR. a reply. Trans. R. Soc Trop Med Hyg. 88, 363.
Black, J., Hommel, M., Snounou, G., and Pinder, M. (1994) Mixed infections with Plasmodium falciparum and P malariae and fever in malaria Lancet 343, 1095.
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© 1996 Humana Press Inc.
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Snounou, G. (1996). Detection and Identification of the Four Malaria Parasite Species Infecting Humans by PCR Amplification. In: Clapp, J.P. (eds) Species Diagnostics Protocols. Methods in Molecular Biology™, vol 50. Humana Press. https://doi.org/10.1385/0-89603-323-6:263
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DOI: https://doi.org/10.1385/0-89603-323-6:263
Publisher Name: Humana Press
Print ISBN: 978-0-89603-323-8
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