Abstract
The last decade has seen an extraordinary improvement in fluorescence microscopy instruments. These have been mainly in the development of epi-illumination systems, including dichroic beam splitters, matching excitation and barrier filters, and special objectives (see ref. 1 for background information). Routinely we use a JenaLumar fluorescence microscope (Zeiss, Jena, Germany) equipped with oil immersion wide field × 100 objective with an adjustable iris diaphragm. According to our experience, the best documentation is obtained when the photographs are taken on Kodak Tri X-Pan film processed in Kodak developer.
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© 1996 Humana Press Inc., Totowa, NJ
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Hašek, J., Streiblová, E. (1996). Fluorescence Microscopy Methods. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:391
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DOI: https://doi.org/10.1385/0-89603-319-8:391
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