Yeast Protocols

Methods in Cell and Molecular Biology

  • Ivor H. Evans

Part of the Methods in Molecular Biology™ book series (MIMB, volume 53)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. John F. T. Spencer, Dorothy M. Spencer
    Pages 1-4
  3. John F. T. Spencer, Dorothy M. Spencer
    Pages 5-15
  4. John F. T. Spencer, Dorothy M. Spencer
    Pages 17-38
  5. John F. T. Spencer, Dorothy M. Spencer
    Pages 39-44
  6. Brendan P. G. Curran, Virginia C. Bugeja
    Pages 45-49
  7. John F. T. Spencer, Dorothy M. Spencer
    Pages 51-58
  8. Carl Saunders-Singer
    Pages 59-67
  9. Ann Vaughan-Martini, Alessandro Martini
    Pages 89-102
  10. Peter Piper
    Pages 103-107
  11. Graham R. Bignell, Angela R. M. Miller, Ivor H. Evans
    Pages 109-116
  12. Barry Panaretou, Peter Piper
    Pages 117-121
  13. Michael A. Quail, Steven L. Kelly
    Pages 123-131
  14. Ben Distel, Inge van der Leij, Wilko Kos
    Pages 133-138
  15. Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield
    Pages 139-145
  16. Stephen A. Johnston, Michael J. DeVit
    Pages 147-153
  17. Graham R. Bignell, Ivor H. Evans
    Pages 155-187
  18. Matthew John Kleinman
    Pages 189-192

About this book

Introduction

Yeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods—for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced—first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.

Editors and affiliations

  • Ivor H. Evans
    • 1
  1. 1.School of Chemical and Life SciencesUniversity of GreenwichLondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033198
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-319-1
  • Online ISBN 978-1-59259-540-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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