Abstract
Reporter gene constructs are used to monitor the expression of genes whose natural product is not readily assayed. The most convenient reporters to use encode enzymes that catalyze a reaction that is easily monitored. Three useful reporter systems for intracellular expression will be described here: β-galactosidase (1), chloramphenicol acetyl transferase (CAT) (2,3), and aminoglycoside phosphotransferase (APT) (4). Each reporter has advantages and disadvantages according to the simplicity or the accuracy of the assay.
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© 1996 Humana Press Inc., Totowa, NJ
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Mount, R.C., Jordan, B.E., Hadfield, C. (1996). Reporter Gene Systems for Assaying Gene Expression in Yeast. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:239
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DOI: https://doi.org/10.1385/0-89603-319-8:239
Publisher Name: Humana Press
Print ISBN: 978-0-89603-319-1
Online ISBN: 978-1-59259-540-2
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