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Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae

  • Protocol
Yeast Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 53))

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Abstract

The development of genetically marked Ty elements that transpose at high levels has made Ty mutagenesis a useful tool in yeast genetics (1,2). Ty mutagenesis is useful for several reasons:

  1. 1.

    Mutations made by insertion of a marked Ty element into a gene permit the rapid cloning of that gene into Escherichia coli.

  2. 2.

    A tagged locus can be rapidly mapped to a chromosome by hybridization to chromosomes separated by electrophoresis.

  3. 3.

    Mutations caused by Ty elements have useful phenotypes.

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Author information

Authors and Affiliations

  1. Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Frederick, MD

    David J. Garfinkel

Authors
  1. David J. Garfinkel
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Editor information

Editors and Affiliations

  1. School of Chemical and Life Sciences, University of Greenwich, London, UK

    Ivor H. Evans

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© 1996 Humana Press Inc., Totowa, NJ

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Garfinkel, D.J. (1996). Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae . In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:227

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  • DOI: https://doi.org/10.1385/0-89603-319-8:227

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-319-1

  • Online ISBN: 978-1-59259-540-2

  • eBook Packages: Springer Protocols

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