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Determination of Plasmid Copy Number in Yeast

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Yeast Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 53))

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Abstract

Autonomously replicative plasmids are widely employed as vectors for the expression of cloned genes in yeast (1). A number of different kinds of such plasmid vectors exist, which fall into three classes: ARS (autonomously replicating sequence) based plasmids, 2-µm-based plasmids, and ARS plasmids containing a centromere.

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References

  1. Romanos, M. A., Scorer, C. A., and Clare, J. J. (1992) Foreign gene expression in yeast a review. Yeast 8, 423–488.

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Author information

Authors and Affiliations

  1. Department of Genetics, University of Leicester, UK

    Bernadette E. Jordan, Robert C. Mount & Christopher Hadfield

Authors
  1. Bernadette E. Jordan
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  2. Robert C. Mount
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  3. Christopher Hadfield
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Editor information

Editors and Affiliations

  1. School of Chemical and Life Sciences, University of Greenwich, London, UK

    Ivor H. Evans

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© 1996 Humana Press Inc., Totowa, NJ

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Jordan, B.E., Mount, R.C., Hadfield, C. (1996). Determination of Plasmid Copy Number in Yeast. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:193

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  • DOI: https://doi.org/10.1385/0-89603-319-8:193

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-319-1

  • Online ISBN: 978-1-59259-540-2

  • eBook Packages: Springer Protocols

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