Abstract
The isolation of membranes from cultured mammalian cells poses a number of problems. First and foremost is the problem of homogenization, which is particularly difficult with suspension culture cells, as opposed to those that grow as an adherent monolayer. The choice of subcellular fractionation procedure depends on the results of the homogenization. For a review of the problems associated with the isolation of plasma membranes from tissue culture cells, see Graham (1).
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References
Graham, J. M. (1982) Preparation of plasma membrane from tissue culture cells, in Cancer Cell Organelles, Methodological Surveys, vol. 11 (Reid, E., Cook, G. M., W., and Morre, D. J., eds.), Ellis Horwood, Chichester, UK, pp. 342–358.
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© 1993 Humana Press Inc.
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Graham, J.M. (1993). Isolation of Membranes from Tissue Culture Cells. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:97
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DOI: https://doi.org/10.1385/0-89603-236-1:97
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-236-1
Online ISBN: 978-1-59259-506-8
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