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Biomembrane Protocols

I. Isolation and Analysis

  • John M. Graham
  • Joan A. Higgins
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 19)

Table of contents

  1. Front Matter
    Pages i-x
    PDF
  2. The Identification of Subcellular Fractions from Mammalian Cells
    John M. Graham
    Pages 1-18
  3. The Isolation of Nuclei and Nuclear Membranes from Rat Liver
    John M. Graham
    Pages 19-28
  4. Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver
    John M. Graham
    Pages 29-40
  5. Continuous-Flow Electrophoresis
    John M. Graham
    Pages 41-49
  6. Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells
    John M. Graham
    Pages 51-57
  7. Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver
    Laura Scott, Michael J. Schell, Ann L. Hubbard
    Pages 59-69
  8. The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients
    Terry C. Ford
    Pages 71-82
  9. The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components
    Antony P. Jackson
    Pages 83-96
  10. Isolation of Membranes from Tissue Culture Cells
    John M. Graham
    Pages 97-108
  11. The Isolation of Membranes from Bacteria
    Robert K. Poole
    Pages 109-122
  12. Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts
    David G. Whitehouse, Anthony L. Moore
    Pages 123-131
  13. Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
    Anthony L. Moore, Anne-Catherine Fricaud, Andrew J. Walters, David G. Whitehouse
    Pages 133-139
  14. Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption
    J. Paul Luzio, Peter J. Richardson
    Pages 141-151
  15. Separation and Analysis of Phospholipids by Thin Layer Chromatography
    Ian J. Cartwright
    Pages 153-167
  16. Analysis of Phospholipids by High Performance Liquid Chromatography
    Hafez A. Ahmed
    Pages 169-177
  17. Measurement of Cholesterol in Membranes
    Hafez A. Ahmed
    Pages 179-182
  18. Analysis of Fatty Acids by Gas Liquid Chromatography
    Ian J. Cartwright
    Pages 183-195
  19. Chemical Assays for Proteins
    David J. Winterbourne
    Pages 197-202
  20. Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis
    Michael J. Dunn, Samantha J. Bradd
    Pages 203-210
  21. Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence
    Samantha J. Bradd, Michael J. Dunn
    Pages 211-218
  22. Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins
    Joe Corbett, Michael J. Dunn
    Pages 219-227
  23. Isolation of a Membrane-Bound Enzyme, 5′-Nucleotidase
    J. Paul Luzio, Elaine M. Bailyes
    Pages 229-242
  24. The Isolation of Membrane Proteoglycans
    Malcolm Lyon
    Pages 243-251
  25. Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans
    Jeremy E. Turnbull
    Pages 253-267
  26. Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration
    Anthony P. Corfield
    Pages 269-286
  27. The Extraction and Analysis of Glycosphingolipids
    Norman A. Gregson
    Pages 287-301
  28. Back Matter
    Pages 303-313
    PDF

About this book

Keywords

Biomembran Peroxisom Western Blotting biology biomembrane enzymes glycoprotein immunology membrane membrane proteins molecular biology protein proteins proteoglycans tissue

Editors and affiliations

  • John M. Graham
    • 1
  • Joan A. Higgins
    • 2
  1. 1.Merseyside Innovation CentreLiverpoolUK
  2. 2.Department of Molecular Biology and BiotechnologyUniversity of SheffieldUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896032361
  • Copyright Information Humana Press 1993
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-236-1
  • Online ISBN 978-1-59259-506-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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