Abstract
The isolation of photosynthetically active chloroplasts is the starting point for many plant metabolic studies as diverse as carbon assimilation, electron flow and phosphorylation, metabolite transport, and protein targeting. Whatever the subsequent use of the chloroplasts, it is preferable to attempt to isolate intact chloroplasts, as the organelle envelope will protect the stroma and thylakoids from the deleterious effects of degradative enzymes and phenolic compounds released from leaf tissues during the preparative procedures.
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© 1993 Humana Press Inc.
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Whitehouse, D.G., Moore, A.L. (1993). Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:123
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DOI: https://doi.org/10.1385/0-89603-236-1:123
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-236-1
Online ISBN: 978-1-59259-506-8
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