Abstract
The chapters in the two volumes of Biomembrane Protocols are broadly devoted to the methodologies involved in the structural and functional characterization of cell membranes. In many cases, a study of a particular membrane presupposes that it can be isolated by disruption of the cells and fractionation of the resulting homogenate, and then identified unambiguously. The early chapters of this volume, Biomembrane Protocols: I. Isolation and Analysis, are concerned with this fractionation process. Later chapters are concerned with the determination of composition, whereas in the second volume, Biomembrane Protocols: II. Architecture and Function, the methods associated with architecture and function predominate.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Glastris, B. and Pfieffer, S. B. (1974) Mammalian membrane marker enzymes: sensitive assay for 5′-nucleotidase and assay for mammalian 2′3′-cyclic nucleotide 3′-phosphohydrolase. Methods Enzymol. 32, 124–131.
Graham, J. M. (1975) Cellular membrane fractionation, in New Techniques in Biophysics and Cell Biology (Pain, R. and Smith, B. J., eds.), Wiley, London, pp. 1–42.
Widnell, C. C. (1974) Purification of rat liver 5′-nucleotidase as a complex with sphingomyelin. Methods Enzymol. 32, 368–374.
Avruch, J. and Wallach, D. F. H. (1972) Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells. Biochim. Biophys. Acta 233, 334–347.
Esmann M. (1988) ATPase and phosphatase activity of Na+/K+-ATPase: molar and specific activity, protein determination. Methods Enzymol. 156, 105–115.
Norby, J. G. (1988) Coupled assay of Na+/K+-ATPase activity. Methods Enzymol. 156, 116–119.
Chang, P. L., Rosa, N. E., and Davidson, R. G. (1981) Differential assay of arylsulfatase A and B activities: a sensitive method for cultured human cells. Anal. Biochem. 117, 382–389.
Mackler, B., Collip, P. S., Duncan, H. M., Rao, N. A., and Huennekens, F. H. (1962) An electron transport particle from yeast: purification and properties. J. Biol. Chem. 237, 2968–2974.
Davis, G. A. and Bloom, F. E. (1973) Subcellular particles separated through a histochemical reaction. Anal. Biochem. 51, 429–435.
Cohen, G., Dembiec, D., and Marcus, J. (1970) Measurement of catalase activity in tissue extracts. Anal. Biochem. 34, 30–38.
Kim, Y. S., Perdomo, J., and Nordberg, J. (1971) Glycoprotein biosynthesis in small intestinal mucosa. I A study of glycosyl transferases in microsomal subfractions. J. Biol. Chem. 246, 5466–5476.
Beaufay, H., Amar-Costesec, A., Feytmans, E., Thines-Sempoux, D., Wibo, M., Robbi, M., and Berthet, J. (1974) Analytical study of microsomes and isolated subcellular fraction from rat liver. J. Cell Biol. 61, 188–200.
Fleischer, B., Fleischer, S., and Ozawa, H. (1969) Isolation and characterization of Golgi membranes from bovine liver. J. Cell Biol. 43, 59–79.
Williams, C. H. and Kamin, H. (1962) Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver. J. Biol. Chem. 237, 587–595.
Aronson, N. N. and Touster, O. (1974) Isolation of rat liver plasma membrane fragments in isotonic sucrose. Methods Enzymol. 31, 90–102.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Humana Press Inc.
About this protocol
Cite this protocol
Graham, J.M. (1993). The Identification of Subcellular Fractions from Mammalian Cells. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:1
Download citation
DOI: https://doi.org/10.1385/0-89603-236-1:1
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-236-1
Online ISBN: 978-1-59259-506-8
eBook Packages: Springer Protocols