Abstract
In biospecific ligand affinity chromatography, the interaction between the immobilized ligand and the solute molecule is based on their complementarity of charge, hydrophobicity, shape, and so on. The same forces that govern these interactions play a role in pseudobiospecific systems, but perhaps differ in their relative magnitude (1).
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References
Vijayalakshmi M. A. (1989) Pseudo-biospecific ligand affinity chromatography. Tibtech 7(3), 71–76.
Lehninger A. L. (1976) Biochemistry. Worth, New York, 230, 231.
Kanoun S., Amourache L., Krishnan S., and Vijayalakshmi M. A. (1986) A new support for the large scale purification of proteins. J. Chromatogr. 376, 259–267.
Hjalmarsson S. and Akesson R. (1983), Modern Kjeldahl procedure. Int. Lab. 70–76.
Chang S. H., Gooding K. M., and Regnier F. E. (1976) Use of oxiranes in die preparation of bonded phase supports. J. Chromatogr. 120, 321–333.
Sundberg L. and Porath J. (1974) Preparation of adsorbents for biospecific affinity chromatography. 1. Attachment of amino group containing ligands to insoluble polymers by means of bifunctional oxiranes. J. Chromatogr. 90, 87–98.
Kanoun S. (1988) Contribution à la chromatographic de pseudo-affinité. Modele de protéines: Les immunoglobulines G placentaires. PhD Thesis, University of Compiegne, 95–99.
Amourache L. and Vijayalakshmi M. A. (1984) Affinity chromatography of kid chymosin on histidyl-sepharose. J Chromatogr. 303, 285–290.
Melin S. and Vijayalakshmi M. A. (1987) Zonal elution analysis of IgG retention on histidyl-sepharose. Biol. Chem. Hoppe-Seyler’s 368(7), 762.
Krishnan S., Gaehel-Vignais I., and Vyayalakshmi M. A. (1987) A semipreparative isolation of carboxypeptidase. Isoenzymes from Aspergillus Niger by a single immobilized metal ion affinity chromatography. J. Chromatogr. 397, 339–346.
Vijayalakshmi M. A. and Thomas D. (1985) Affinity chromatography as a production technique. The World Biotech. Report Online 1 (Europe), 131–140.
Cabrera K. E. and Wilchek M. (1987) Coupling of ligands to primary hydroxyl containing silica for high performance affinity chromatography. J Chromatogr. 397, 187–196.
Akoum A., Vijayalakshmi M. A., Cardon P., Fournet B., Sigot M., and Guespin-Michel J. F. (1987) Myxococcus xanthus produces an extracellular glycopeptide diat displays blood anticoagulant properties. Enz. Microb. Technol. 9, 426–429.
Akoum A., Vijayalakshmi M. A., and Sigot M. (1989) Scale-up of “Myxalme” purification by a pseudo-affinity method using a radial flow column. Chromatographic 28, 157–160.
Elkak A. and Vijayalakshmi M. A. (1991) Study of die separation of mouse monoclonal antibodies by pseudobioaffinity chromatography using matrixlinked histidine and histamine. J. Chromatogr. 570, 29–41.
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© 1992 Humana Press Inc., Totowa, NJ
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Vijayalakshmi, M.A. (1992). Histidine Ligand Affinity Chromatography. In: Kenney, A., Fowell, S. (eds) Practical Protein Chromatography. Methods in Molecular Biology™, vol 11. Humana Press. https://doi.org/10.1385/0-89603-213-2:33
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DOI: https://doi.org/10.1385/0-89603-213-2:33
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