Practical Protein Chromatography

  • Andrew Kenney
  • Susan Fowell
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 11)

Table of contents

  1. Front Matter
    Pages i-x
    PDF
  2. Thiophilic Adsorption Chromatography
    T. William Hutchens
    Pages 1-15
  3. Immobilized Metal Ion Affinity Chromatography
    Tai-Tung Yip, T. William Hutchens
    Pages 17-31
  4. Histidine Ligand Affinity Chromatography
    Mookambeswaran A. Vijayalakshmi
    Pages 33-44
  5. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes
    James H. Hageman, Glenn D. Kuehn
    Pages 45-71
  6. Calcium-Dependent Hydrophobic Interaction Chromatography
    Nicholas H. Battey, Michael A. Venis
    Pages 73-80
  7. Lectin Affinity Chromatography
    Iris West, Owen Goldring
    Pages 81-89
  8. Dye-Ligand Affinity Chromatography
    Steven J. Burton
    Pages 91-103
  9. High-Performance Affinity Chromatography for Protein Separation and Purification
    Yannis D. Clonis
    Pages 105-123
  10. Immunoafflnity Chromatography
    George W. Jack
    Pages 125-133
  11. Selection of Antibodies for Immunoaffinity Chromatography
    Duncan S. Pepper
    Pages 135-171
  12. Some Alternative Coupling Chemistries for Affinity Chromatography
    Duncan S. Pepper
    Pages 173-196
  13. Exploiting Weak Affinities
    Sten Ohlson
    Pages 197-208
  14. Biospecific Affinity Elution
    Robert Kerry Scopes
    Pages 209-221
  15. Size-Exclusion High-Performance Liquid Chromatography of Proteins
    David A. Harris
    Pages 223-236
  16. Chromatofocusing
    Chee Ming Li, T. William Hutchens
    Pages 237-248
  17. Ion-Exchange Chromatography of Proteins
    Andrew C. Kenney
    Pages 249-258
  18. Displacement Chromatography of Proteins
    Steven M. Cramer
    Pages 259-271
  19. Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography
    David Hornby, Kevin Ford, Paul Shore
    Pages 273-286
  20. Making Bacterial Extracts Suitable for Chromatography
    Roger F. Sherwood
    Pages 287-305

About this book

Editors and affiliations

  • Andrew Kenney
    • 1
  • Susan Fowell
    • 2
  1. 1.Drew Scientific LimitedLondon
  2. 2.Leicester PolytechnicScraptoftUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896032132
  • Copyright Information Humana Press 1992
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-213-2
  • Online ISBN 978-1-59259-498-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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