Abstract
Proteins, blotted from polyacrylamide gels onto nitrocellulose sheets (Western Blots) can be stained nonspecifically with a variety of dyes, or they can be identified individually by probing with appropriate antibodies. These procedures may be performed on duplicate blots, staining the total protein pattern on one blot and using the second blot for the immune reaction (1,2). This chapter describes how to combine both methods on one blot, i.e., staining the blot first for total protein, followed by an indirect immune reaction (3).
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References
Rohringer, R. and Holden, D. W. (1985) Protein blotting: Detection of protein with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes. Anal. Biochem. 144, 118–127.
Hancock, K. and Tsang, V. C. W. (1983) India ink staining of proteins on nitrocellulose paper. Anal. Biochem. 133, 157–162.
Egger, D. and Bienz, K. (1987) Colloidal gold staining and immunoprobing of proteins on the same nitrocellulose blot. Anal. Biochem. 166, 413–417.
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© 1992 The Humana Press, Inc., Totowa, NJ
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Egger, D., Bienz, K. (1992). Colloidal Gold Staining and Immunoprobing on the Same Western Blot. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:247
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DOI: https://doi.org/10.1385/0-89603-204-3:247
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
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