Overview
- Editors:
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Margaret M. Manson
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Medical Research Council Laboratories, Carshalton, UK
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Table of contents (50 protocols)
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Front Matter
Pages i-xiii
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- Jonathan A. Green, Margaret M. Manson
Pages 1-5
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- Boguslaw Szewczyk, Donald F. Summers
Pages 7-12
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- Monique Diano, André Le Bivic, Michel Hirn
Pages 13-22
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- David C. Hancock, Gerard I. Evan
Pages 23-32
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- David C. Hancock, Gerard I. Evan
Pages 33-41
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- Yveline Frobert, Jacques Grassi
Pages 65-78
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- Michael G. Baines, Robin Thorpe
Pages 79-104
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- Michael G. Ormerod, Susanne F. Imrie
Pages 117-124
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- Jonathan A. Green, Margaret M. Manson
Pages 125-129
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- Jonathan A. Green, Richard E. Edwards, Margaret M. Manson
Pages 131-135
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- Edward A. Bayer, Meir Wilchek
Pages 137-142
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- Edward A. Bayer, Meir Wilchek
Pages 143-148
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- Edward A. Bayer, Meir Wilchek
Pages 149-162
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About this book
Molecular biologists can no longer afford to be experts in only one par ticular area. They need to be equally competent at handling DNA, RNA, and proteins, moving from one area to another as required by the problem. Ad vances in the field have been greatly helped by numerous applications of antibody-antigen interactions, so that a basic knowledge of immunology has now become useful. The emphasis of Immunochemical Protocols is not on the production of antibodies, since many people will beg or buy these, but rather on the enor mous range of applications to which these powerful reagents can be put. For completeness, however, methods are included for the production of both polyclonal and monoclonal antibodies. Techniques that may be helpful in obtaining antibodies to elusive antigens and to synthetic peptides are also provided. Both qualitative and quantitative methods are described for local izing, characterizing, and quantifying various antigens. These range from the use of antibodies in screening DNA libraries to their application in EUSA assays for quantifying adducts in alkylated DNA Each method is described by an author who has regularly used the tech in his or her own laboratory. Not all the techniques necessarily repre nique sent the state-of-the-art-they are, however, all dependable methods that regularly achieve the desired result. Each chapter starts with a brief description of the basic theory behind the technique being described. The main aim of this book, however, is to outline the practical steps necessary for carrying out the method successfully.
Reviews
Beyond question...the book will be used in many laboratories....[A] fine addition to the...library of publications on immunological methods.-Molecular Immunology
Editors and Affiliations
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Medical Research Council Laboratories, Carshalton, UK
Margaret M. Manson