Abstract
Ressler first described in 1960 (1) a form of immunoelectrophoresis now called crossed immunoelectrophoresis (CIE) or two-dimensional immunoelectrophoresis, which was later improved by Laurell (2), Clarke and Freeman (3), and Weeke (4), among others. CIE is superior to the classical immunoelectrophoresis, according to Grabar and Williams (5), particularly in providing better resolution and quantitative capabilities. It combines the electrophoretic separation of the sample proteins in agarose gel with electrophoresis at right angles to the initial separation in an antibody-containing agarose. Each protein separated during the first dimension forms a separate precipitation peak during the second dimension. Moreover, the area under any protein peak is directly proportional to the concentration of that protein in the analyzed sample and inversely proportional to the concentration of antibody to that protein in the antiserum used.
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References
Ressler, N. (1960) Two dimensional electrophoresis of protein-antigens with an antibody-containing buffer. Gin. Chim. Acta 5, 795–800.
Laurell, C. B. (1965) Antigen antibody crossed electrophoresis. Anal. Biochem. 10, 358–361.
Clarke H. G. M. and Freeman T. (1967) Quantitative immunoelectrophoresis method (Laurell electrophoresis), in Protides of the Biological Fluids, 14th Colloquium, 1966 (Peeters, H., ed.), Elsevier, Amsterdam, pp. 503–509.
Weeke, B. (1970) Carbamylated human transferrin used as a reference in the Laurell crossed electrophoresis. Scand. J. Clin Lab. Invest. 25, 161–163.
Grabar, P. and Williams, C. A. (1953) Méthode permettant l’étude conjuguée des 1. propriétés électrophorétiques et immunochimiques d’un mélange de protéines: Application au sérum sanguin. Biochim. Biophys. Acta 10, 193.
Emmett, M. and Crowle, A. J. (1982) Crossed immunoelectrophoresis: Qualitative and quantitative considerations. J. Immunol Methods 50, R65–R83.
Davril, M., Laine, A., and Hayem, A. (1987) Studies on the interactions of human pancreatic elastase 2 with human γ1-proteinase inhibitor and γ1-antichymotrypsin. Biochem J. 245, 699–704.
Kroll, J. (1968) Quantitative immunoelectrophoresis. Scand. J. Clin. Lab. Invest. 22, 112–114.
Svendsen, P. J. and Axelsen, N. H. A. (1972) A modified antigen-antibody crossed electrophoresis characterizing the specificity and titre of human precipitins against Candida albicans. J. Immuncl Methods 1, 169–176.
Platt, H. S., Sewell, B. M., Feldman, T., and Souhami, R. L. (1973) Further modifications of the two-dimensional electrophoretic technique of Laurell. Clin. Chim. Acta 46, 419–429.
Laine, A., Ducourouble, M. P., and Hannothiaux, M. H. (1987) Identification of proteins by crossed immunoelectrophoresis with a trap-gel. Anal Biochem. 161, 39–44.
Krøll, J. (1968) On the immunoelectrophoretical identification and quantitation of serum proteins. Scand. J. Clin. Lab. Invest. 22, 79–81.
Krøll, J. (1969) Immunochemical identification of specific precipitin lines in quantitative immunoelectrophoresis patterns. Scand. J. Clin, Lab. Invest. 24, 55–60.
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© 1992 The Humana Press, Inc., Totowa, NJ
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Laine, A. (1992). Crossed Immunoelectrophoresis. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:207
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DOI: https://doi.org/10.1385/0-89603-204-3:207
Publisher Name: Humana Press, Totowa, NJ
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