Abstract
The rocket immunoelectrophoresis technique or electroimmunodiffusion (EID) (1) is a simple, fast, and reproducible technique for quantitation of a single protein, and is also applicable in a protein mixture. Several unknown samples can be analyzed on a single plate. Known reference solutions have to be included in each plate. To obtain an accurate quantitation, the proteins in the reference solutions and in the unknown samples have to be physicochemically and immunologically identical. The samples are applied in wells punched out of an agarose gel containing the corresponding monospecific antiserum. One-dimensional electrophoresis is performed and rocket-shaped precipitates are formed. The quantitation is based on measuring the height of the precipitate peak.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Laurell, C. B. (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15, 45–52.
Mancini, G., Carbonara, A. O., and Heremans J. F. (1965) Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–254.
Krøll, J. Jensen, K. A., and Lyngbye, J. (1970) Quantitative immunoelectrophoresis as routine analysis, in Methods in Clinical Qiemistry, Vol. 1. Karger, Basel/München/Paris/New York, pp. 131–139.
Laurell, C. B. (1972) Electroimmunoassay. Scand. J. Clin. Lab. Invest. 29(Suppl. 124), 21–37.
Davril, M., Laine, A., and Hayem, A. (1987) Studies on the interactions of human pancreatic elastase 2 with human α1-proteinase inhibitor and α1-antichymotrypsin. Biochem. J. 245, 699–704.
Hansen, S. A. (1988) Immunostaining of rocket immunoelectrophoresis precipitates too weak for identification following staining with Coomassie Brilliant Blue. Electrophoresis 9, 101–102.
Laurell, C. B. and McKay, E. J. (1981) Electroimmunoassy, in Methods in Enzymology (Langone, J. J. and Van Vunakis, H., eds.), Academic, London, pp. 339–369.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 The Humana Press, Inc., Totowa, NJ
About this protocol
Cite this protocol
Laine, A. (1992). Rocket Immunoelectrophoresis Technique or Electroimmunodiffusion. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:201
Download citation
DOI: https://doi.org/10.1385/0-89603-204-3:201
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
eBook Packages: Springer Protocols