Abstract
In immunoelectron microscopy (IEM), simultaneous labeling of two or more antigens on the same section is desirable for many applications. If the antibodies (Ab) to be used are raised in the same species, as is usually the case with monoclonal antibodies (MAb), the difficulty arises that the labeled secondary, antispecies Ab used in the first labeling step traps the primary Ab directed against the second antigen, thus leading to a nonspecific signal for the second antigen.
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References
Bienz, K., Egger, D., and Pasamontes, L. (1986) Electron microscopic immunocytochemistry: Silver enhancement of colloidal gold marker allows double labeling with the same primary antibody. J. Histochem. Cytochem. 34, 1337–1342.
Kopriwa, B. M. (1975) A comparison of various procedures for fine grain development in electron microscopic radioautography. Histochemistry 44, 201–224.
Bienz, K (1977) Techniques and applications of autoradiography in the light and electron microscope. Microsc. Ada 79, 1–22.
Holgate, C. S., Jackson, P., Cowen, P. N., and Bird, C. C. (1983) Immunogold-silver staining: New method of immunostaining with enhanced sensitivity. J. Histochem. Cytochem. 31, 938–944.
Moeremans, M., Daneels, G., van Dijck, A., Langanger, G., and De Mey, J. (1984) Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. J. Immunol Meth 74, 353–360.
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© 1992 The Humana Press, Inc., Totowa, NJ
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Bienz, K., Egger, D. (1992). Electron Microscopic Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:187
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DOI: https://doi.org/10.1385/0-89603-204-3:187
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
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