Skip to main content

Chemical Modification of Proteins

  • Protocol
New Protein Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 3))

  • 1533 Accesses

Abstract

The two most widely used applications for chemical modification are in primary structure analysis and in the identification of essential groups involved in the binding and catalytic sites of proteins. The methods discussed here are those used frequently in primary structure analysis. Chemical modifications involving protein “active center” identification are the subject of a review by Pfleiderer (1).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Pfleiderer, G. (1985) Chemical Modification of Proteins in Modern Methods in Protein Chemistry, Review Articles vol. 2 (Tschesche, H., ed.) Walter de Gruyter, Berlin.

    Google Scholar 

  2. Hewick, R.M., Hunkapiller, M.W., Hood, L.E., and Dreyer, W. J. (1981) A gas-liquid-solid-phase peptide and protein sequenator. J. Biol. Chem. 256, 7990–7997.

    PubMed  CAS  Google Scholar 

  3. Glazer, A.N., Delange, R.J., and Sigman, D.S. (1975) Chemical Characterization of Proteins and Their Derivatives, in Chemical Modification of Proteins (Work, T.S., and Work, E., eds.) North Holland/American Elsevier, Amsterdam.

    Google Scholar 

  4. Wang, S.S. and Carpenter, F.H. (1968) Kinetic studies at high pH of the trypsin-catalyzed hydrolysis of N-alphabenzoyl derivatives of L-arginamide, L-lysinamide and S-2-aminoethyl-L-cyteinamide and related compounds. J. Biol. Chem. 243, 3702–3710.

    PubMed  CAS  Google Scholar 

  5. Slobin, L. I. and Singer, S.J. (1968) The specific cleavage of immunoglobulin polypeptide chains at cysteinyl residues. J. Biol. Chem. 243, 1777–1786.

    PubMed  CAS  Google Scholar 

  6. Levy, M., Fishman, L., and Schenkein, I. (1970) Mouse Submaxillary Gland Proteases, in Methods in Enzymology vol. XIX Proteolytic Enzymes (Perlmann, G.E. and Lorand, L., eds.) Academic, New York.

    Google Scholar 

  7. Mitchell, W.M. and Harrington, W.F. (1970) Clostripain, im Methods in Enzymology vol. XIX Proteolytic Enzymes (Perlmann, G.E. and Lorand, L., eds.) Academic, New York.

    Google Scholar 

  8. Thomas, K.A. and Bradshaw, R.A. (1981) γ-Subunit of Mouse Submaxillary Gland 7S Nerve Growth Factor: An Endopeptidase of the Serine Family, in Methods in Enzymology, vol. 80, Proteolytic Enzymes, part C (Lorand, L., ed.) Academic, New York.

    Google Scholar 

  9. Schroeder, W.A., Shelton, J.R., and Robberson, B. (1967) Modification of methionyl residues during amino-ethylation. Biochem. Biophys. Acta 147, 590–592.

    PubMed  CAS  Google Scholar 

  10. Shotton, D. and Hartley, B.S. (1973) Evidence for the amino acid sequence of porcine pancreatic elastase. Biochem. J. 131, 643–675.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1988 The Humana Press Inc.

About this protocol

Cite this protocol

Carne, A.F. (1988). Chemical Modification of Proteins. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:89

Download citation

  • DOI: https://doi.org/10.1385/0-89603-126-8:89

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-126-5

  • Online ISBN: 978-1-59259-490-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics