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New Protein Techniques

  • Book
  • © 1988

Overview

Editors:
  1. John M. Walker

    1. The Hatfield Polytechnic, Hatfield, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 3)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Prevention of Unwanted Proteolysis

    • Robert J. Beynon
    Pages 1-23

  3. The Bradford Method for Protein Quantitation

    • John B. W. Hammond, Nicholas J. Kruger
    Pages 25-32

  4. Amino Acid Analysis by Precolumn Derivatization

    • E. L. V. Harris
    Pages 33-47

  5. Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography

    • E. L. V. Harris
    Pages 49-55

  6. Enzymatic Methods for Cleaving Proteins

    • Bryan John Smith
    Pages 57-69

  7. Chemical Cleavage of Proteins

    • Bryan John Smith
    Pages 71-88

  8. Chemical Modification of Proteins

    • Alex F. Carne
    Pages 89-98

  9. The Design, Preparation, and Use of Immunopurification Reagents

    • Andrew Kenney, Lynne Goulding, Christopher Hill
    Pages 99-110

  10. Dye-Ligand Chromatography

    • Sarojani Angal
    Pages 111-122

  11. Aminohexyl-Sepharose Affinity Chromatography

    • R. D. J. Barker, H. M. Bailey
    Pages 123-134

  12. Purification of DNA-Dependent RNA Polymerase from Eubacteria

    • N. W. Scott
    Pages 135-147

  13. Direct Immunoprecipitation of Protein

    • Christopher F. Thurston, Lucy F. Henley
    Pages 149-158

  14. Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique

    • Ketan Patel, David J. Easty, Michael J. Dunn
    Pages 159-168

  15. Chromatofocusing

    • Ake Sidén, Paolo Gallo
    Pages 169-186

  16. Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels

    • Michael J. Dunn, Ketan Patel
    Pages 187-201

  17. Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension

    • Michael J. Dunn, Ketan Patel
    Pages 203-216

  18. Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension

    • Michael J. Dunn, Ketan Patel
    Pages 217-231

  19. Preparative Aspects of Immobilized pH Gradients

    • Pier Giorgio Righetti, Cecilia Gelfi
    Pages 233-256

  20. Isoelectric Focusing Under Denaturing Conditions

    • Christopher F. Thurston, Lucy F. Henley
    Pages 257-267
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Editors and Affiliations

  • The Hatfield Polytechnic, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: New Protein Techniques

  • Editors: John M. Walker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896031268

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1988

  • Hardcover ISBN: 978-0-89603-126-5Published: 30 August 1988

  • Softcover ISBN: 978-1-4899-4381-1Published: 18 August 2013

  • eBook ISBN: 978-1-59259-490-0Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 531

  • Number of Illustrations: 33 b/w illustrations

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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