Abstract
Most immunochemical techniques used in molecular biology rely on a specific class of antibodies, immunoglobulin G (IgG). In mammals, IgG antibodies are produced during the secondary humoral response and contribute about 80% of the serum immunoglobulin. Factors affecting the experimental production of antibodies have been discussed in detail (1). In this chapter we describe a method for the rapid and efficient purification of IgG to near homogeneity from rabbit serum. This is achieved by affinity chromatography using protein A.
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© 1988 The Humana Press Inc.
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Kruger, N.J., Hammond, J.B.W. (1988). Purification of Immunoglobulins Using Protein A-Sepharose. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:363
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DOI: https://doi.org/10.1385/0-89603-126-8:363
Publisher Name: Humana Press
Print ISBN: 978-0-89603-126-5
Online ISBN: 978-1-59259-490-0
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