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Measuring Recombination Proficiency in Mouse Embryonic Stem Cells

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Molecular Toxicology Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1105))

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Abstract

A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break (DSB). A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a DSB, the repair of which is also quantifiable.

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References

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Author information

Authors and Affiliations

  1. Cell Biology Program, Memorial Sloan-Kettering Cancer Center, Cornell University Graduate School of Medical Sciences, 1275 York Avenue, New York, NY, 10021, USA

    Maria Jasin

  2. Markey Cancer Center, University of Kentucky, Lexington, KY, USA

    Andrew J. Pierce

Authors
  1. Andrew J. Pierce
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  2. Maria Jasin
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Corresponding author

Correspondence to Maria Jasin .

Editor information

Editors and Affiliations

  1. Occupational Health, University of Pittsburgh Dept. Environmental &, Pittsburgh, Pennsylvania, USA

    Phouthone Keohavong

  2. Dept. of Pharmaceutical Sciences, Nova Southeastern University College of Pharmacy, Fort Lauderdale, Florida, USA

    Stephen G. Grant

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© 2014 Humana Press

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Pierce, A.J., Jasin, M. (2014). Measuring Recombination Proficiency in Mouse Embryonic Stem Cells. In: Keohavong, P., Grant, S. (eds) Molecular Toxicology Protocols. Methods in Molecular Biology, vol 1105. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-739-6_34

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  • DOI: https://doi.org/10.1007/978-1-62703-739-6_34

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-738-9

  • Online ISBN: 978-1-62703-739-6

  • eBook Packages: Springer Protocols

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