Abstract
The transfer and immobilization of biological macromolecules onto solid nitrocellulose or nylon (polyvinylidene difluoride (PVDF)) membranes subsequently followed by specific detection is referred to as blotting. DNA blots are called Southerns after the inventor of the technique, Edwin Southern. By analogy, RNA blots are referred to as northerns and protein blots as westerns (Burnette, Anal Biochem 112:195–203, 1981). With few exceptions, western blotting involves five steps, namely, sample collection, preparation, separation, immobilization, and detection. In this chapter, protocols for the entire process from sample collection to detection are described.
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References
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Petrasovits, L.A. (2014). Protein Blotting Protocol for Beginners. In: Henry, R., Furtado, A. (eds) Cereal Genomics. Methods in Molecular Biology, vol 1099. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-715-0_16
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DOI: https://doi.org/10.1007/978-1-62703-715-0_16
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-714-3
Online ISBN: 978-1-62703-715-0
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