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  • Book
  • © 2014

Cereal Genomics

Methods and Protocols

  • Is a comprehensive resource for studying cereal genomes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1099)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-x
  2. RNA Extraction from Cereal Vegetative Tissue

    • Julie A. Pattemore
    Pages 17-21
  3. cDNA Library Preparation

    • Maarten Kooiker, Gang-Ping Xue
    Pages 29-40
  4. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants

    • Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng, Song Weining
    Pages 41-63
  5. The Polymerase Chain Reaction (PCR): General Methods

    • Daniel L. E. Waters, Frances M. Shapter
    Pages 65-75
  6. Mutation and Mutation Screening

    • L. Slade Lee, Bradley J. Till, Helen Hill, Owen A. Huynh, Joanna Jankowicz-Cieslak
    Pages 77-95
  7. Genome Walking

    • Frances M. Shapter, Daniel L. E. Waters
    Pages 133-146
  8. Functional Analysis by Protein Biochemistry

    • Louis M. T. Bradbury
    Pages 147-158
  9. Genomic Southern Blot Analysis

    • Leigh Gebbie
    Pages 159-177
  10. Protein Blotting Protocol for Beginners

    • Lars A. Petrasovits
    Pages 189-199
  11. Genetic Transformation of Wheat via Particle Bombardment

    • Caroline A. Sparks, Huw D. Jones
    Pages 201-218
  12. Sorghum Genetic Transformation by Particle Bombardment

    • Guoquan Liu, Bradley C. Campbell, Ian D. Godwin
    Pages 219-234
  13. Genetic Transformation of Wheat via Agrobacterium-Mediated DNA Delivery

    • Caroline A. Sparks, Angela Doherty, Huw D. Jones
    Pages 235-250

About this book

​In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

Editors and Affiliations

  • Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Australia

    Robert J. Henry

  • Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Lismore, Australia

    Agnelo Furtado

Bibliographic Information

  • Book Title: Cereal Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Robert J. Henry, Agnelo Furtado

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-715-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-714-3Published: 16 November 2013

  • Softcover ISBN: 978-1-4939-6039-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-715-0Published: 19 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 300

  • Number of Illustrations: 10 b/w illustrations, 29 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Transgenics

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access