Abstract
Immunofluorescence microscopy is a widely used laboratory method which allows detection and visualization of specific antigens. The method employs the specificity of antibodies to deliver fluorophore to a specific target and then visualize it with a microscope. The power of the technique is that it requires relatively little manipulation and relatively few bacterial cells, enabling the detection of antigen expression where other methods cannot, such as during an actual infection in an animal. Here, we apply the method to follow antigen expression on the surface of MRSA cells over time in in vivo infection models.
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Disclosure and Acknowledgements
All authors are employees of Pfizer and as such may also own stock in the company. This work was funded by Pfizer. We would like to thank Drs Paul Liberator and Bruce Green, both of Pfizer, for their helpful reviews of this chapter.
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Timofeyeva, Y., Scully, I.L., Anderson, A.S. (2014). Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin-Resistant Staphylococcus aureus (MRSA). In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 1085. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-664-1_4
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DOI: https://doi.org/10.1007/978-1-62703-664-1_4
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Publisher Name: Humana Press, Totowa, NJ
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