Abstract
Noncoding RNAs are attractive targets for molecular recognition because of the central role they play in gene expression. Since most noncoding RNAs are in a double-helical conformation, recognition of such structures is a formidable problem. Herein, we describe a method for sequence-selective recognition of biologically relevant double-helical RNA (illustrated on ribosomal A-site RNA) using peptide nucleic acids (PNA) that form a triple helix in the major grove of RNA under physiologically relevant conditions. Protocols for PNA preparation and binding studies using isothermal titration calorimetry are described in detail.
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Acknowledgement
This work was supported by NIH grant GM071461 and Binghamton University.
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Zengeya, T., Gupta, P., Rozners, E. (2014). Sequence Selective Recognition of Double-Stranded RNA Using Triple Helix-Forming Peptide Nucleic Acids. In: Nielsen, P., Appella, D. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 1050. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-553-8_7
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DOI: https://doi.org/10.1007/978-1-62703-553-8_7
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Publisher Name: Humana Press, Totowa, NJ
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