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Cellular Delivery of Peptide Nucleic Acids (PNAs)

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Peptide Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1050))

Abstract

Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)–PNA conjugates and transfection of PNA–ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.

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Acknowledgments

This work was supported by the Lundbeck Foundation and the Danish Council for Independent Research/Medical Sciences.

Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark

    Takehiko Shiraishi

  2. Department of Cellular and Molecular Medicine, University of Copenhagen, Faculty of Health Sciences, Copenhagen, Denmark

    Peter E. Nielsen

Authors
  1. Takehiko Shiraishi
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  2. Peter E. Nielsen
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Editor information

Editors and Affiliations

  1. Dept. Cellular & Molecular Medicine, University of Copenhagen Faculty of Health Sciences, Copenhagen N, Denmark

    Peter E Nielsen

  2. NIH NIDDK, Bethesda, USA

    Daniel H. Appella

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Shiraishi, T., Nielsen, P.E. (2014). Cellular Delivery of Peptide Nucleic Acids (PNAs). In: Nielsen, P., Appella, D. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 1050. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-553-8_16

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  • DOI: https://doi.org/10.1007/978-1-62703-553-8_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-552-1

  • Online ISBN: 978-1-62703-553-8

  • eBook Packages: Springer Protocols

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