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In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

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Genotoxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1044))

Abstract

Chromosome damage is a very important indicator of genetic damage relevant to environmental and clinical studies. Detailed descriptions of the protocols used for detection of chromosomal aberrations induced by unknown agents in vitro both in the presence or the absence of rat liver-derived metabolizing systems are given. Structural chromosomal aberrations that can be observed and quantified at metaphases are described here. For the detection of chromosomal damage (fragments or whole chromosome) in interphase, the micronucleus test can be used and a description of this test is also presented. Criteria for determining a positive result using appropriate statistical methods are described.

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Authors and Affiliations

  1. Dipartimento di Scienze Ecologiche e Biologiche, Università degli Studi della Tuscia, Largo dell’Università, Viterbo, Italy

    Pasquale Mosesso, Adyapalam T. Natarajan & Fabrizio Palitti

  2. Research Toxicology Centre, Pomezia (Roma), Italy

    Serena Cinelli

Authors
  1. Pasquale Mosesso
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  2. Serena Cinelli
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  3. Adyapalam T. Natarajan
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  4. Fabrizio Palitti
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Editor information

Editors and Affiliations

  1. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Alok Dhawan

  2. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Mahima Bajpayee

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Mosesso, P., Cinelli, S., Natarajan, A.T., Palitti, F. (2013). In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 1044. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-529-3_6

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  • DOI: https://doi.org/10.1007/978-1-62703-529-3_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-528-6

  • Online ISBN: 978-1-62703-529-3

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