Genotoxicity Assessment

Methods and Protocols

  • Alok Dhawan
  • Mahima Bajpayee

Part of the Methods in Molecular Biology book series (MIMB, volume 1044)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Gene Mutation Assays

    1. Front Matter
      Pages 1-1
    2. Errol Zeiger
      Pages 3-26
    3. Melissa R. Schisler, Martha M. Moore, B. Bhaskar Gollapudi
      Pages 27-50
    4. Jeffrey C. Bemis, Nikki E. Hall, Stephen D. Dertinger
      Pages 51-77
    5. Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, Robert H. Heflich
      Pages 79-95
    6. Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, Robert H. Heflich
      Pages 97-119
  3. Assays for Chromosomal Abnormalities

    1. Front Matter
      Pages 121-121
    2. Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, Fabrizio Palitti
      Pages 123-146
    3. Francesca Pacchierotti, Valentina Stocchi
      Pages 147-163
    4. Christian Johannes, Guenter Obe
      Pages 165-178
    5. Sawako Kasamoto, Shoji Masumori, Makoto Hayashi
      Pages 179-189
    6. Claudia Bolognesi, Michael Fenech
      Pages 191-207
    7. Azeddine Elhajouji, Magdalena Lukamowicz-Rajska
      Pages 209-235
    8. Ilse Decordier, Micheline Kirsch-Volders
      Pages 237-244
  4. Tests for Primary DNA Damage

    1. Front Matter
      Pages 291-291
    2. Eugenia Cordelli, Lorena Paris
      Pages 293-310
    3. Julian Laubenthal, Michal R. Gdula, Alok Dhawan, Diana Anderson
      Pages 311-323

About this book

Introduction

Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.

Keywords

Comet assay technique DNA DNA synthesis and 32P--post labelling Drosophila Genotoxicity Assessment ICH guidelines cytogenetic techniques genotoxicity assessment of chemicals in vitro in bacteria mouse lymphoma primary DNA damage test--Pig--a gene mutation transgenic animals

Editors and affiliations

  • Alok Dhawan
    • 1
  • Mahima Bajpayee
    • 2
  1. 1.School of Science and TechnologyAhmedabad University Institute of Life SciencesAhmedabadIndia
  2. 2.School of Science and TechnologyAhmedabad University Institute of Life SciencesAhmedabadIndia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-529-3
  • Copyright Information Springer Science+Business Media, New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-528-6
  • Online ISBN 978-1-62703-529-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Chemical Manufacturing
Biotechnology
Consumer Packaged Goods
Pharma