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The Comet Assay in Marine Animals

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Genotoxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1044))

Abstract

Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions, when applied in marine animals.

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Author information

Authors and Affiliations

  1. Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

    Giada Frenzilli

  2. Weymouth Laboratory, CEFAS, Weymouth, Dorset, UK

    Brett P. Lyons

Authors
  1. Giada Frenzilli
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  2. Brett P. Lyons
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Editor information

Editors and Affiliations

  1. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Alok Dhawan

  2. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Mahima Bajpayee

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© 2013 Springer Science+Business Media, New York

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Frenzilli, G., Lyons, B.P. (2013). The Comet Assay in Marine Animals. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 1044. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-529-3_19

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  • DOI: https://doi.org/10.1007/978-1-62703-529-3_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-528-6

  • Online ISBN: 978-1-62703-529-3

  • eBook Packages: Springer Protocols

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