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Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides

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Cyclic Nucleotide Signaling in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1016))

Abstract

Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular.

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Author information

Authors and Affiliations

  1. Division of Chemical and Life Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia

    Natalia Maria Ordoñez & Chris Gehring

  2. School of Agricultural Science, University of Tasmania, Hobart, TAS, Australia

    Lana Shabala & Sergey Shabala

Authors
  1. Natalia Maria Ordoñez
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  2. Lana Shabala
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  3. Chris Gehring
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  4. Sergey Shabala
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Editors and Affiliations

  1. , Division of Chemical and Life Sciences a, 4700 King Abdullah University of Science, N/A, Thuwal, 23955-6900, Saudi Arabia

    Chris Gehring

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Ordoñez, N.M., Shabala, L., Gehring, C., Shabala, S. (2013). Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides. In: Gehring, C. (eds) Cyclic Nucleotide Signaling in Plants. Methods in Molecular Biology, vol 1016. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-441-8_7

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  • DOI: https://doi.org/10.1007/978-1-62703-441-8_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-440-1

  • Online ISBN: 978-1-62703-441-8

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