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Protocol for the Preparation of Stimuli-Responsive Gold Nanoparticles Capped with Elastin-Based Pentapeptides

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Cellular and Subcellular Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 991))

Abstract

Stimuli-responsive materials are playing an increasingly important role in a wide range of applications such as drug delivery, diagnostics, sensors, and tissue engineering. Among them, gold nanoparticles responding to changes in their surrounding environment are of particular interest due to their size-related optical properties. Here, we present a novel strategy for the preparation of gold nanoparticles exhibiting a stimuli-responsive behavior. We rely on the use of a ligand consisting of only a single repeat of the elastin-based pentapeptide VPGVG. In this contribution, we describe a protocol for the solid-phase peptide synthesis of thiol-terminated VPGVG ligand, and for the preparation of gold nanoparticles covered with the pentapeptide through a ligand-exchange reaction.

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Acknowledgments

The authors thank the Netherlands Organisation for Scientific Research (NWO) and the Fonds québécois de la recherche sur la nature et les technologies (FQRNT) for their financial support. Christine Lavigueur is acknowledged for the design of Fig. 1.

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Authors and Affiliations

  1. Department of Organic Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands

    Vincent Lemieux, P. Hans H. M. Adams & Jan C. M. van Hest

Authors
  1. Vincent Lemieux
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  2. P. Hans H. M. Adams
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  3. Jan C. M. van Hest
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Editor information

Editors and Affiliations

  1. College of Pharmacy, Dept. Pharmaceutical Sciences, Midwestern University, N. 59th Avenue 19555, Glendale, 85308, Arizona, USA

    Volkmar Weissig

  2. , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, N. 59th Avenue 19555, Glendale, 85308, Arizona, USA

    Tamer Elbayoumi

  3. , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, N. 59th Avenue 19555, Glendale, 85308, Arizona, USA

    Mark Olsen

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Lemieux, V., Adams, P.H.H.M., van Hest, J.C.M. (2013). Protocol for the Preparation of Stimuli-Responsive Gold Nanoparticles Capped with Elastin-Based Pentapeptides. In: Weissig, V., Elbayoumi, T., Olsen, M. (eds) Cellular and Subcellular Nanotechnology. Methods in Molecular Biology, vol 991. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-336-7_32

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  • DOI: https://doi.org/10.1007/978-1-62703-336-7_32

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-335-0

  • Online ISBN: 978-1-62703-336-7

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